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Literature summary extracted from

  • Doun, S.S.; Burgner, J.W.2nd.; Briggs, S.D.; Rodwell, V.W.
    Enterococcus faecalis phosphomevalonate kinase (2005), Protein Sci., 14, 1134-1139.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.4.2 medicine the enzymes of the mevalonate pathway of isopentenyl diphosphate biosynthesis represent potential for addressing a pressing human health concern, the development of antibiotics against resistent strains of the Gram-positive streptococci Enterococcus faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.2 expression in Escherichia coli BL21(DE3) Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.2 0.17
-
 ATP
-
 Enterococcus faecalis
2.7.4.2 0.19
-
 (R,S)-5-phosphomevalonate
-
 Enterococcus faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.2 Ca2+ 10 mM cannot replace Mn2+ or Mg2+ Enterococcus faecalis
2.7.4.2 Mn2+ preferred cation to activate the enzyme, optimal concentration about 4 mM Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.2 40500
-
 2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence Enterococcus faecalis
2.7.4.2 48000
-
 2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence Enterococcus faecalis
2.7.4.2 76000
-
 analytical ultracentrifugation Enterococcus faecalis
2.7.4.2 81000
-
 calculated from amino acid sequence Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate Enterococcus faecalis mevalonate pathway of isopentenyl diphosphate biosynthesis ADP + (R)-5-diphosphomevalonate
-
 ?
2.7.4.2 ATP + (R)-5-phosphomevalonate Enterococcus faecalis 41 mevalonate pathway of isopentenyl diphosphate biosynthesis ADP + (R)-5-diphosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.2 Enterococcus faecalis
-
-
-
2.7.4.2 Enterococcus faecalis 41
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.2 recombinant protein Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.2 3.9
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate mevalonate pathway of isopentenyl diphosphate biosynthesis Enterococcus faecalis ADP + (R)-5-diphosphomevalonate
-
 ?
2.7.4.2 ATP + (R)-5-phosphomevalonate mevalonate pathway of isopentenyl diphosphate biosynthesis Enterococcus faecalis 41 ADP + (R)-5-diphosphomevalonate
-
 ?
2.7.4.2 ATP + (R,S)-5-phosphomevalonate
-
 Enterococcus faecalis ADP + (R,S)-5-diphosphomevalonate
-
 ?
2.7.4.2 ATP + (R,S)-5-phosphomevalonate
-
 Enterococcus faecalis 41 ADP + (R,S)-5-diphosphomevalonate
-
 ?
2.7.4.2 CTP + (R,S)-5-phosphomevalonate 0.4% activity Enterococcus faecalis CDP + (R,S)-5-diphosphomevalonate
-
 ?
2.7.4.2 CTP + (R,S)-5-phosphomevalonate 0.4% activity Enterococcus faecalis 41 CDP + (R,S)-5-diphosphomevalonate
-
 ?
2.7.4.2 GTP + (R,S)-5-phosphomevalonate 3.6% activity Enterococcus faecalis GDP + (R,S)-5-diphosphomevalonate
-
 ?
2.7.4.2 GTP + (R,S)-5-phosphomevalonate 3.6% activity Enterococcus faecalis 41 GDP + (R,S)-5-diphosphomevalonate
-
 ?
2.7.4.2 TTP + (R,S)-5-phosphomevalonate 1.6% activity Enterococcus faecalis TDP + (R,S)-5-diphosphomevalonate
-
 ?
2.7.4.2 TTP + (R,S)-5-phosphomevalonate 1.6% activity Enterococcus faecalis 41 TDP + (R,S)-5-diphosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.2 dimer 2 * 48000, SDS-PAGE, analytical ultracentrifugation, 2 * 40500, amino acid sequence Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.2 phosphomevalonate kinase
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.2 37
-
-
 Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.2 8
-
-
 Enterococcus faecalis