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Literature summary extracted from

  • Nesbitt, N.M.; Baleanu-Gogonea, C.; Cicchillo, R.M.; Goodson, K.; Iwig, D.F.; Broadwater, J.A.; Haas, J.A.; Fox, B.G.; Booker, S.J.
    Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase (2005), Protein Expr. Purif., 39, 269-282.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.181 KHCO3 100 mM, slight activation Escherichia coli
2.3.1.181 NaOAc 100 mM, slight activation Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.181 N-terminal hexahistidine-tagged apo-H protein Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.181 octanoyl-CoA 0.5 mM, 67% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.181 0.0102
-
 octanoyl-[acyl-carrier protein] pH 7.2, 37°C Escherichia coli
2.3.1.181 0.0132
-
 apo-H protein pH 7.2, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.181 10000
-
 monomer, gel filtration Escherichia coli
2.3.1.181 29000
-
 trimer, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.181 octanoyl-[acyl-carrier protein] + apo-H protein Escherichia coli LipB reaction represents the first committed step in the biosynthesis of the lipoyl cofactor. apo-H protein is the lipoyl bearing subunit of the glycine cleavage system APO H-protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.181 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.181 N-terminal hexahistidine-tagged apo-H protein Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.181 0.54
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.181 octanoyl-[acyl-carrier protein] + apo-H protein LipB reaction represents the first committed step in the biosynthesis of the lipoyl cofactor. apo-H protein is the lipoyl bearing subunit of the glycine cleavage system Escherichia coli APO H-protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?
2.3.1.181 octanoyl-[acyl-carrier protein] + apo-H protein apo-H protein is the lipoyl bearing subunit of the glycine cleavage system Escherichia coli apo-H protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.181 LipB
-
 Escherichia coli
2.3.1.181 lipoyl(octanoyl)transferase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.181 0.2
-
 octanoyl-[acyl-carrier protein] pH 7.2, 37°C Escherichia coli
2.3.1.181 0.2
-
 apo-H protein pH 7.2, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.181 7.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.181 6 9 pH 6.0: about 65% of maximal activity, pH 9.0: about 40% of maximal activity Escherichia coli