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Literature summary extracted from

  • Linster, C.L.; Van Schaftingen, E.
    A spectrophotometric assay of D-glucuronate based on Escherichia coli uronate isomerase and mannonate dehydrogenase (2004), Protein Expr. Purif., 37, 352-360.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.12 overexpression in Escherichia coli BL21(DE3)pLysS Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.12 EDTA 0.25 mM, 50% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.12 0.29
-
 D-glucuronate in absence of added metal or chelator Escherichia coli
5.3.1.12 0.31
-
 D-glucuronate in presence of 0.01 mM Zn2+ Escherichia coli
5.3.1.12 0.41
-
 D-glucuronate in presence of 0.01 mM Co2+ Escherichia coli
5.3.1.12 1.2
-
 D-glucuronate in presence of 0.1 mM Mn2+ Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.12 Co2+ stimulation is more potent at lower concentrations than stimulation by Mn2+ Escherichia coli
5.3.1.12 Mn2+ maximal stimulation is higher than stimulatipn by Zn2+ or Co2+, stimulation at low concentrations is lower than stimulation by Zn2+ and Co2+ Escherichia coli
5.3.1.12 Zn2+ stimulation is more potent at lower concentrations than stimulation by Mn2+. Vmax is about 3times higher in presence of 0.1 mM Mn2+ than in presence of 0.01 mM Zn2+ or Co2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.12
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.12 D-Glucuronate
-
 Escherichia coli D-Fructuronate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.12 Uronate isomerase
-
 Escherichia coli