EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.11.1 | gene pknI, DNA and amino acid sequence determination and analysis, NaCl-inducible expression of N-terminally His-tagged full-length and cytoplasmic domain of PknI in Escherichia coli strain BL21 in inclusion bodies | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.11.1 | cytoplasm | enzyme contains a cytoplasmic domain | Mycobacterium tuberculosis | 5737 | - |
2.7.11.1 | membrane | - |
Mycobacterium tuberculosis | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.1 | Ca2+ | can partly substitute for Mn2+, activates | Mycobacterium tuberculosis | |
2.7.11.1 | Mg2+ | activates | Mycobacterium tuberculosis | |
2.7.11.1 | Mn2+ | strictly required, activates | Mycobacterium tuberculosis | |
2.7.11.1 | additional information | no activity with Mg2+ alone, no activity with Cu2+ or Zn2+ | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.11.1 | 55000 | - |
x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE | Mycobacterium tuberculosis |
2.7.11.1 | 61805 | - |
x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.1 | Mycobacterium tuberculosis | - |
- |
- |
2.7.11.1 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.1 | recombinant His-tagged full-length and cytoplasmic domain of PknI from Escherichia coli strain BL21 by nickel affinity chromatography | Mycobacterium tuberculosis |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.7.11.1 | refolding of recombinant enzyme | Mycobacterium tuberculosis |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.11.1 | ? | x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.11.1 | PknI | - |
Mycobacterium tuberculosis |
2.7.11.1 | serine/threonine protein kinase | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.1 | 37 | - |
assay at | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.1 | 7.4 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.1 | ATP | - |
Mycobacterium tuberculosis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.11.1 | Mycobacterium tuberculosis | amino acid sequence calculation | - |
5.5 |