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Literature summary extracted from

  • Lane, S.B.; Tchedre, K.T.; Nair, M.P.; Thigpen, A.E.; Lacko, A.G.
    Characterization of lecithin:cholesterol acyltransferase expressed in a human lung cell line (2004), Protein Expr. Purif., 36, 157-164.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.43 apolipoprotein A-I
-
 Homo sapiens

Application

EC Number Application Comment Organism
2.3.1.43 medicine the recombinant enzyme is utilized for enzyme replacement therapy in case of congenital enzyme deficiency Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.43 optimization of expression in human lung cell line H1299, enzyme is secreted to the culture medium Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.43 0.00056
-
 1,2-bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphocholine fluorescent assay, pH 7.4, 25°C, recombinant enzyme Homo sapiens
2.3.1.43 0.0006
-
 phosphatidylcholine radioassay, pH 7.4, 37°C, recombinant enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.43 extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 44000
-
 x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE Homo sapiens
2.3.1.43 64000
-
 x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.43 additional information Homo sapiens enzyme deficiency can cause corneal opacity, proteinuria, anemia, and kidney failure ?
-
 ?
2.3.1.43 phosphatidylcholine + cholesterol Homo sapiens
-
 1-acylglycerophosphocholine + cholesteryl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens P04180
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.43 glycoprotein analysis of sugar composition, overview Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 recombinant enzyme from human lung cell line H1299 culture medium Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.43 liver
-
 Homo sapiens
-
2.3.1.43 plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 additional information
-
 activities produced by different lung cell clones expressing the enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 1,2-bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphocholine + cholesterol fluorescent substrate assay Homo sapiens ?
-
 ?
2.3.1.43 additional information enzyme deficiency can cause corneal opacity, proteinuria, anemia, and kidney failure Homo sapiens ?
-
 ?
2.3.1.43 phosphatidylcholine + cholesterol
-
 Homo sapiens 1-acylglycerophosphocholine + cholesteryl ester
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.43 ? x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.43 LCAT
-
 Homo sapiens
2.3.1.43 lecithin:cholesterol acyltransferase
-
 Homo sapiens