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Literature summary extracted from

  • Ochiai, A.; Mori, S.; Kawai, S.; Murata, K.
    Overexpression, purification, and characterization of ATP-NAD kinase of Sphingomonas sp. A1 (2004), Protein Expr. Purif., 36, 124-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23
-
 Sphingomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 HgCl2 1 mM, 6% residual activity Sphingomonas sp.
2.7.1.23 additional information not inhibitory: NADH Sphingomonas sp.
2.7.1.23 NADP+ 0.1 mM, 38% residual activity Sphingomonas sp.
2.7.1.23 NADPH 0.1 mM, 67% residual activity Sphingomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.43
-
 NAD+ 30°C, pH 8.0 Sphingomonas sp.
2.7.1.23 1.92
-
 ATP 30°C, pH 8.0 Sphingomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.23 CaCl2 46% of activity with MgCl2 Sphingomonas sp.
2.7.1.23 CoCl2 31% of activity with MgCl2 Sphingomonas sp.
2.7.1.23 MgCl2
-
 Sphingomonas sp.
2.7.1.23 MnCl2 329% of activity with MgCl2 Sphingomonas sp.
2.7.1.23 ZnCl2 231% of activity with MgCl2 Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 32000
-
 2 * 32000, SDS-PAGE Sphingomonas sp.
2.7.1.23 64000
-
 gel filtration Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Sphingomonas sp. Q6L7J5 expression in Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.23 0.281
-
 30°C, pH 8.0 Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+
-
 Sphingomonas sp. ADP + NADP+
-
 ?
2.7.1.23 CTP + NAD+ 37% of the activity with ATP Sphingomonas sp. CDP + NADP+
-
 ?
2.7.1.23 dATP + NAD+ 58% of the activity with ATP Sphingomonas sp. dADP + NADP+
-
 ?
2.7.1.23 GTP + NAD+ 100% of the activity with ATP Sphingomonas sp. GDP + NADP+
-
 ?
2.7.1.23 TTP + NAD+ 19% of the activity with ATP Sphingomonas sp. TDP + NADP+
-
 ?
2.7.1.23 UTP + NAD+ 48% of the activity with ATP Sphingomonas sp. UDP + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 dimer 2 * 32000, SDS-PAGE Sphingomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.23 50 55
-
 Sphingomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.23 63
-
 10 min, 50% residual activity Sphingomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.23 2.95
-
 NAD+ 30°C, pH 8.0 Sphingomonas sp.
2.7.1.23 30.33
-
 ATP 30°C, pH 8.0 Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.23 8
-
-
 Sphingomonas sp.