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Literature summary extracted from
- Engineering the substrate specificity of xylose isomerase (2004), Protein Eng. Des. Sel., 17, 861-869.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.5 | expression in Escherichia coli | Actinoplanes missouriensis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.1.5 | - |
Actinoplanes missouriensis |
| 5.3.1.5 | - |
Streptomyces rubiginosus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.1.5 | F26W | catalytic efficiency with L-arabinose is increased 2fold | Actinoplanes missouriensis |
| 5.3.1.5 | Q256D | catalytic efficiency with L-arabinose is increased 3fold, reaction rate with L-ribose is increased 6fold | Actinoplanes missouriensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.5 | 900 | - |
L-arabinose | mutant Q256D | Actinoplanes missouriensis |  |
| 5.3.1.5 | 1400 | - |
L-arabinose | mutant F26W | Actinoplanes missouriensis | |
| 5.3.1.5 | 1500 | - |
L-arabinose | wild-type | Actinoplanes missouriensis | |
| 5.3.1.5 | 1600 | - |
D-glucose | mutant F26W | Actinoplanes missouriensis | |
| 5.3.1.5 | 1900 | - |
D-glucose | wild-type | Actinoplanes missouriensis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.5 | D-Xylose | Actinoplanes missouriensis | - |
D-Xylulose | - |
? | |
| 5.3.1.5 | D-Xylose | Streptomyces rubiginosus | - |
D-Xylulose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.5 | Actinoplanes missouriensis | - |
- |
- |
| 5.3.1.5 | Streptomyces rubiginosus | P24300 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.5 | - |
Actinoplanes missouriensis |
| 5.3.1.5 | - |
Streptomyces rubiginosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.5 | D-Glucose | - |
Actinoplanes missouriensis | D-Fructose | - |
? | |
| 5.3.1.5 | D-Glucose | - |
Streptomyces rubiginosus | D-Fructose | - |
? | |
| 5.3.1.5 | D-Xylose | - |
Actinoplanes missouriensis | D-Xylulose | - |
? | |
| 5.3.1.5 | D-Xylose | - |
Streptomyces rubiginosus | D-Xylulose | - |
? | |
| 5.3.1.5 | L-arabinose | - |
Actinoplanes missouriensis | L-ribulose | - |
r | |
| 5.3.1.5 | L-arabinose | low reaction efficiency | Streptomyces rubiginosus | L-ribulose | - |
r | |
| 5.3.1.5 | L-ribose | no increased reaction rate observed for mutant F26W | Actinoplanes missouriensis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.5 | tetramer | alpha4, crystal structure analysis | Streptomyces rubiginosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.5 | XI | - |
Actinoplanes missouriensis |
| 5.3.1.5 | XI | - |
Streptomyces rubiginosus |
| 5.3.1.5 | xylose isomerase | - |
Actinoplanes missouriensis |
| 5.3.1.5 | xylose isomerase | - |
Streptomyces rubiginosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | additional information | - |
75-80°C for mutant F26W | Actinoplanes missouriensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 65 | - |
mutant Q256D remains fully active for 60 min at 65°C, whereas the activity is rapidly lost at 80°C | Actinoplanes missouriensis |
| 5.3.1.5 | 75 | - |
wild-type enzyme remains fully active for 60 min at 75°C | Actinoplanes missouriensis |
| 5.3.1.5 | 80 | - |
mutant F26W remains fully active for 60 min at 80°C | Actinoplanes missouriensis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.5 | 0.1 | - |
L-arabinose | wild-type | Actinoplanes missouriensis | |
| 5.3.1.5 | 0.155 | - |
L-arabinose | mutant F26W | Actinoplanes missouriensis | |
| 5.3.1.5 | 0.158 | - |
L-arabinose | mutant Q256D | Actinoplanes missouriensis | |
| 5.3.1.5 | 20.5 | - |
D-glucose | mutant F26W | Actinoplanes missouriensis | |
| 5.3.1.5 | 25.3 | - |
D-glucose | wild-type | Actinoplanes missouriensis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 7.6 | - |
- |
Actinoplanes missouriensis |
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Release 2023.1 (January 2023)
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