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Literature summary extracted from

  • Chang, H.Y.; Huang, H.C.; Lee, J.H.; Peng, H.L.
    Characterization of a putative Pseudomonas UDPglucose pyrophosphorylase (1999), Proc. Natl. Sci. Counc. Repub. China Part B Life Sci., 23, 74-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9 expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.115
-
 diphosphate
-
 Pseudomonas aeruginosa
2.7.7.9 0.83
-
 D-glucose-1-phosphate
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 30943
-
 4 * 30943, SDS-PAGE Pseudomonas aeruginosa
2.7.7.9 130000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
2.7.7.9 -70°C, 50% glycerol, 2 months, no significant loss of activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 additional information no utilization of the nucleotide diphosphate sugars UDP-Gal, UDP-GlnA, UDP-Man, UDP-Xyl, ADP-Glc, CDP-Glc and TDP-Glc Pseudomonas aeruginosa ?
-
 ?
2.7.7.9 UTP + D-glucose-1-phosphate
-
 Pseudomonas aeruginosa UDP-glucose + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.9 tetramer 4 * 30943, SDS-PAGE Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.9 35 45 mutant pHC3007 Pseudomonas aeruginosa