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Literature summary extracted from

  • Sitrit, Y.; Ninio, R.; Bar, E.; Golan, E.; Larkov, O.; Ravid, U.; Lewinsohn, E.
    S-Linalool synthase activity in developing fruit of the columnar cactus koubo [Cereus peruvianus (L.) Miller] (2004), Plant Sci., 167, 1257-1262.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.25 DTT
-
 Cereus peruvianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.25 0.0056
-
 Mn2+ cofactor Mn2+, pH 6.6, 30°C Cereus peruvianus
4.2.3.25 0.018
-
 geranyl diphosphate pH 6.6, 30°C Cereus peruvianus
4.2.3.25 0.06
-
 Mg2+ cofactor Mg2+, pH 6.6, 30°C Cereus peruvianus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.25 Mg2+
-
 Cereus peruvianus
4.2.3.25 Mn2+ preferred cation Cereus peruvianus
4.2.3.25 additional information divalent cation is required for activity, no activity with Cu2+, Co2+, and Ca2+, in presence of Fe2+ spontaneous enzyme-independent formation of racemic linalool from geranyl diphosphate occurs Cereus peruvianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.25 53000
-
 gel filtration Cereus peruvianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.25 geranyl diphosphate + H2O Cereus peruvianus enantio-specific reaction, the enzyme plays a pivotal role in production of flavor and aroma of ripe cactus fruits (3S)-linalool + diphosphate product identification ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.25 Cereus peruvianus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.25 partially from fruits by anion exchange chromatography Cereus peruvianus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.25 fruit ripening, very low levels in green immature fruits, enzyme levels increase during fruit ripening Cereus peruvianus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.25 geranyl diphosphate + H2O enantio-specific reaction, the enzyme plays a pivotal role in production of flavor and aroma of ripe cactus fruits Cereus peruvianus (3S)-linalool + diphosphate product identification ?
4.2.3.25 geranyl diphosphate + H2O enantio-specific reaction, 85% optical puritiy of S-linalool Cereus peruvianus (3S)-linalool + diphosphate product identification ?
4.2.3.25 additional information spontaneous enzyme-independent formation of racemic linalool from geranyl diphosphate in presence of Fe2+ Cereus peruvianus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.25 30
-
 small scale assay at Cereus peruvianus
4.2.3.25 34
-
 large scale assay at Cereus peruvianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.25 6.6
-
 small scale assay at Cereus peruvianus
4.2.3.25 6.9
-
 large scale assay at Cereus peruvianus