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Literature summary extracted from

  • Michels, A.K.; Wedel, N.; Kroth, P.G.
    Diatom plastids possess a phosphoribulokinase with an altered regulation and no oxidative pentose phosphate pathway (2005), Plant Physiol., 137, 911-920.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.19 dithiothreitol reduced form Trieres chinensis
2.7.1.19 thioredoxin reduced form, regulates activity via reversible reduction Trieres chinensis
2.7.1.19 thioredoxin reduced form, regulates activity via reversible reduction, probably not relevant in vivo Spinacia oleracea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.19 expressed in Escherichia coli Trieres chinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.19 5,5'-dithiobis(2-nitrobenzoic acid) DTNB, Ellman’s reagent, almost complete inhibition at 0.0045 mM Trieres chinensis
2.7.1.19 Cu2+
-
 Trieres chinensis
2.7.1.19 dithiothreitol oxidized form Trieres chinensis
2.7.1.19 glutathione oxidized form Trieres chinensis
2.7.1.19 additional information inhibited by addition of strong oxidants, reversible by addition of reducing agents Trieres chinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.19 0.084
-
 ATP pH 8 Trieres chinensis
2.7.1.19 0.118
-
 D-ribulose 5-phosphate pH 8 Trieres chinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.19 chloroplast
-
 Trieres chinensis 9507
-
2.7.1.19 chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 44000
-
 2 * 44000, SDS-PAGE, native mass by gel filtration Trieres chinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.19 ATP + D-ribulose 5-phosphate Trieres chinensis part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms ADP + D-ribulose 1,5-bisphosphate
-
 ?
2.7.1.19 ATP + D-ribulose 5-phosphate Spinacia oleracea part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms ADP + D-ribulose 1,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Spinacia oleracea P09559
-
-
2.7.1.19 Trieres chinensis Q95033
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19 from chloroplasts Spinacia oleracea
2.7.1.19 recombinant enzyme Trieres chinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.19 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.19 300
-
 purified enzyme, pH 8 Trieres chinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 ATP + D-ribulose 5-phosphate
-
 Trieres chinensis ADP + D-ribulose 1,5-bisphosphate
-
 ?
2.7.1.19 ATP + D-ribulose 5-phosphate
-
 Spinacia oleracea ADP + D-ribulose 1,5-bisphosphate
-
 ?
2.7.1.19 ATP + D-ribulose 5-phosphate part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms Trieres chinensis ADP + D-ribulose 1,5-bisphosphate
-
 ?
2.7.1.19 ATP + D-ribulose 5-phosphate part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms Spinacia oleracea ADP + D-ribulose 1,5-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.19 dimer 2 * 44000, SDS-PAGE, native mass by gel filtration Trieres chinensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.19 10
-
 stable for at least 2 h Trieres chinensis
2.7.1.19 25
-
 unstable when assay is performed at 25°C Trieres chinensis