Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Akashi, T.; Aoki, T.; Ayabe, S.
    Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis (2005), Plant Physiol., 137, 882-891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.105
-
 Glycyrrhiza echinata
4.2.1.105
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.105 0.029
-
 2,7-dihydroxy-4'-methoxyisoflavanone pH 7.5, 30°C Glycine max
4.2.1.105 0.058
-
 2,7-dihydroxy-4'-methoxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
4.2.1.105 0.114
-
 2,7,4'-trihydroxyisoflavanone pH 7.5, 30°C Glycine max
4.2.1.105 0.17
-
 2,5,7,4'-tetrahydroxyisoflavanone pH 7.5, 30°C Glycine max
4.2.1.105 0.21
-
 2,7,4'-trihydroxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
4.2.1.105 0.304
-
 2,5,7,4'-tetrahydroxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.105 Glycine max Q5NUF3 expressed in Escherichia coli
-
4.2.1.105 Glycyrrhiza echinata Q5NUF4 expressed in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.105 2,5,7,4'-tetrahydroxyisoflavanone
-
 Glycine max genistein + H2O
-
 ?
4.2.1.105 2,5,7,4'-tetrahydroxyisoflavanone activity is 0.5% of the activity with 2,7-dihydroxy-4'-methoxyisoflavanone Glycyrrhiza echinata genistein + H2O
-
 ?
4.2.1.105 2,7,4'-trihydroxyisoflavanone activity is 0.5% of the activity with 2,7-dihydroxy-4'-methoxyisoflavanone Glycyrrhiza echinata daidzein + H2O
-
 ?
4.2.1.105 2,7,4'-trihydroxyisoflavanone activity is 40% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone Glycine max daidzein + H2O
-
 ?
4.2.1.105 2,7-dihydroxy-4'-methoxyisoflavanone
-
 Glycyrrhiza echinata formononetin + H2O
-
 ?
4.2.1.105 2,7-dihydroxy-4'-methoxyisoflavanone activity is 15% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone Glycine max formononetin + H2O
-
 ?
4.2.1.105 p-nitrophenyl butyrate activity is 0.6% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone Glycine max ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.105 0.12
-
 2,7,4'-trihydroxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
4.2.1.105 0.19
-
 2,5,7,4'-tetrahydroxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
4.2.1.105 1.6
-
 2,7-dihydroxy-4'-methoxyisoflavanone pH 7.5, 30°C Glycine max
4.2.1.105 5.3
-
 2,7,4'-trihydroxyisoflavanone pH 7.5, 30°C Glycine max
4.2.1.105 9.8
-
 2,7-dihydroxy-4'-methoxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
4.2.1.105 18.1
-
 2,5,7,4'-tetrahydroxyisoflavanone pH 7.5, 30°C Glycine max