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Literature summary extracted from

  • Ralston, L.; Subramanian, S.; Matsuno, M.; Yu, O.
    Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast using soybean type I and type II chalcone isomerases (2005), Plant Physiol., 137, 1375-1388.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.6 expression in bacteria or Saccharomyces cerevisiae Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.6 0.002
-
 4,2',4',6'-Tetrahydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.007
-
 4,2',4',6'-Tetrahydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.01
-
 2'-hydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.013
-
 4,2'-dihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.015
-
 4,2',4',6'-Tetrahydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.015
-
 4,2',5'-trihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.02
-
 4,2',4'-trihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.02
-
 4,2'-dihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.021
-
 2'-hydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.021
-
 2',4'-dihydroxy-4-methylchalcone pH 8, 20°C Glycine max
5.5.1.6 0.029
-
 4,2',5'-trihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.03
-
 4,2',4'-trihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.042
-
 2',4'-dihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 0.049
-
 2',4'-dihydroxy-4-methylchalcone pH 8, 20°C Glycine max
5.5.1.6 0.15
-
 2',4'-dihydroxychalcone pH 8, 20°C Glycine max

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.6 Glycine max Q53B70
-
-
5.5.1.6 Glycine max Q53B74
-
-
5.5.1.6 Glycine max Q93XE6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.6 recombinant Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.6 2',4'-dihydroxy-4-methylchalcone
-
 Glycine max 7-hydroxy-2-(4-methyl-phenyl)-chroman-4-one
-
 ?
5.5.1.6 2',4'-dihydroxychalcone
-
 Glycine max 7-hydroxy-2-phenyl-chroman-4-one
-
 ?
5.5.1.6 2'-hydroxychalcone
-
 Glycine max 2-phenyl-chroman-4-one
-
 ?
5.5.1.6 4,2',4',6'-tetrahydroxychalcone
-
 Glycine max 5,7-dihydroxy-2-(4-hydroxy-phenyl)-chroman-4-one
-
 ?
5.5.1.6 4,2',4'-trihydroxychalcone
-
 Glycine max 7-hydroxy-2-(4-hydroxy-phenyl)-chroman-4-one
-
 ?
5.5.1.6 4,2',5'-trihydroxychalcone
-
 Glycine max 6-hydroxy-2-(4-hydroxy-phenyl)-chroman-4-one
-
 ?
5.5.1.6 4,2'-dihydroxychalcone
-
 Glycine max 2-(4-hydroxy-phenyl)-chroman-4-one
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.6 CHI1A
-
 Glycine max
5.5.1.6 CHI1B2
-
 Glycine max
5.5.1.6 CHI2
-
 Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.6 22.6
-
 4,2'-dihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 32.5
-
 4,2',5'-trihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 35.5
-
 2',4'-dihydroxy-4-methylchalcone pH 8, 20°C Glycine max
5.5.1.6 37.4
-
 2'-hydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 42
-
 2',4'-dihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 51.3
-
 4,2',4'-trihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 57.4
-
 4,2'-dihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 59.9
-
 2'-hydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 62.5
-
 4,2',5'-trihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 84.5
-
 4,2',4'-trihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 86.3
-
 2',4'-dihydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 96.8
-
 2',4'-dihydroxy-4-methylchalcone pH 8, 20°C Glycine max
5.5.1.6 152.2
-
 4,2',4',6'-Tetrahydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 219.1
-
 4,2',4',6'-Tetrahydroxychalcone pH 8, 20°C Glycine max
5.5.1.6 478.8
-
 4,2',4',6'-Tetrahydroxychalcone pH 8, 20°C Glycine max