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Literature summary extracted from

  • Sauter, M.; Cornell, K.A.; Beszteri, S.; Rzewuski, G.
    Functional analysis of methylthioribose kinase genes in plants (2004), Plant Physiol., 136, 4061-4071.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.100 additional information strong upregulation of enzyme expression during sulfur starvation Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.100 DNA and amino acid sequence determination and analysis Arabidopsis thaliana
2.7.1.100 DNA and amino acid sequence determination and analysis, overvexpression of a slightly modified enzyme in Escherichia coli strain BL21 as GST-fusion protein Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.100 additional information insertional mutagenesis results in inability of the plants to grow on methylthioadenosine as asupplemntal sulfur source, knockout plants show unimpaired growth under standard conditions Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.100 additional information
-
 additional information cooperative kinetics Oryza sativa
2.7.1.100 0.0168
-
 S-methyl-5-thio-D-ribose pH 8.5, 37°C Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.100 Mg2+
-
 Oryza sativa
2.7.1.100 Mg2+
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.100 48600
-
 x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE Oryza sativa
2.7.1.100 50000
-
 x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.100 ATP + S-methyl-5-thio-D-ribose Oryza sativa enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
 ?
2.7.1.100 ATP + S-methyl-5-thio-D-ribose Arabidopsis thaliana enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
 ?
2.7.1.100 additional information Oryza sativa the enzyme regulation is related to the sulfur metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.100 Arabidopsis thaliana Q9C6D2
-
-
2.7.1.100 Oryza sativa Q7XR61
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.100 recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off Oryza sativa
2.7.1.100 recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.100 leaf
-
 Arabidopsis thaliana
-
2.7.1.100 root strong upregulation of enzyme expression during sulfur starvation Oryza sativa
-
2.7.1.100 shoot strong upregulation of enzyme expression during sulfur starvation Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.100 ATP + S-methyl-5-thio-D-ribose
-
 Oryza sativa ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
 ?
2.7.1.100 ATP + S-methyl-5-thio-D-ribose
-
 Arabidopsis thaliana ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
 ?
2.7.1.100 ATP + S-methyl-5-thio-D-ribose enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle Oryza sativa ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
 ?
2.7.1.100 ATP + S-methyl-5-thio-D-ribose enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle Arabidopsis thaliana ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
 ?
2.7.1.100 additional information the enzyme regulation is related to the sulfur metabolism Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.100 ? x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
2.7.1.100 methylthioribose kinase
-
 Oryza sativa
2.7.1.100 methylthioribose kinase
-
 Arabidopsis thaliana
2.7.1.100 MTK
-
 Arabidopsis thaliana
2.7.1.100 MTK1
-
 Oryza sativa
2.7.1.100 MTR kinase
-
 Arabidopsis thaliana
2.7.1.100 MTR kinase1
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.100 37
-
 assay at Oryza sativa
2.7.1.100 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.100 8.5
-
 assay at Oryza sativa
2.7.1.100 8.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.100 ATP
-
 Oryza sativa
2.7.1.100 ATP
-
 Arabidopsis thaliana