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Literature summary extracted from

  • Johansson, M.; Mackenzie-Hose, A.; Andersson, I.; Knorpp, C.
    Structure and mutational analysis of a plant mitochondrial nucleoside diphosphate kinase. Identification of residues involved in serine phosphorylation and oligomerization (2004), Plant Physiol., 136, 3034-3042.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expressed as His-tag fusion protein in Escherichia coli DH5alpha Pisum sativum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 H117A activity almost completely abolished Pisum sativum
2.7.4.6 H117D/S119A activity almost completely abolished Pisum sativum
2.7.4.6 S119A reduced serine phosphorylation, only modest decrease of activity Pisum sativum
2.7.4.6 S69A 6% and 14% of wild type activity using dCDP or dTDP as substrate respectively, changes in subunit composition with increasing number of tetramers and dimers Pisum sativum
2.7.4.6 S69A/S119A activity almost completely abolished Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.6 0.07
-
dTDP S69A mutant enzyme, 22°C Pisum sativum
2.7.4.6 0.08
-
dTDP S119A mutant enzyme, 22°C Pisum sativum
2.7.4.6 0.09
-
dCDP S69A mutant enzyme, 22°C Pisum sativum
2.7.4.6 0.1
-
dTDP wild type enzyme, 22°C Pisum sativum
2.7.4.6 0.2
-
dCDP wild type enzyme, 22°C Pisum sativum
2.7.4.6 0.28
-
dCDP S119A mutant enzyme, 22°C Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 mitochondrion
-
Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+ divalent cation required for catalysis, Mg2+ preferred over other ions Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + NDP Pisum sativum contributes to the maintenance of the cellular pools of all nucleosides triphosphates, broad substrate specificity, in plants involved in different signaling pathways ADP + NTP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Pisum sativum Q9SP13 L. cv Oregon sugarpod
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 recombinant protein using His-tag Pisum sativum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.6 50
-
H117A mutant enzyme with dCDP as substrate, 22°C Pisum sativum
2.7.4.6 60
-
S69A/S119A mutant enzyme with dCDP as substrate, 22°C Pisum sativum
2.7.4.6 70
-
H117D/S119A mutant enzyme with dCDP as substrate, 22°C Pisum sativum
2.7.4.6 160
-
S69A mutant enzyme with dCDP as substrate, 22°C Pisum sativum
2.7.4.6 700
-
S69A mutant enzyme with dTDP as substrate, 22°C Pisum sativum
2.7.4.6 2400
-
S119A mutant enzyme with dCDP as substrate, 22°C Pisum sativum
2.7.4.6 2700
-
wild type enzyme with dCDP as substrate, 22°C Pisum sativum
2.7.4.6 3900
-
S119A mutant enzyme with dTDP as substrate, 22°C Pisum sativum
2.7.4.6 5100
-
wild type enzyme with dTDP as substrate, 22°C Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + dCDP
-
Pisum sativum ADP + dCTP
-
?
2.7.4.6 ATP + dTDP
-
Pisum sativum ADP + dTTP
-
?
2.7.4.6 ATP + NDP
-
Pisum sativum ADP + NTP
-
?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates, broad substrate specificity, in plants involved in different signaling pathways Pisum sativum ADP + NTP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 hexamer monomers are arranged as trimers of dimers, crystal structure analysis Pisum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.6 9
-
dCDP S69A mutant enzyme, 22°C Pisum sativum
2.7.4.6 17
-
dTDP S69A mutant enzyme, 22°C Pisum sativum
2.7.4.6 105
-
dCDP S119A mutant enzyme, 22°C Pisum sativum
2.7.4.6 106
-
dCDP wild type enzyme, 22°C Pisum sativum
2.7.4.6 120
-
dTDP S119A mutant enzyme, 22°C Pisum sativum
2.7.4.6 147
-
dTDP wild type enzyme, 22°C Pisum sativum