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Literature summary extracted from

  • Saito, S.; Hirai, N.; Matsumoto, C.; Ohigashi, H.; Ohta, D.; Sakata, K.; Mizutani, M.
    Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid (2004), Plant Physiol., 134, 1439-1449.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.137 expression in baculovirus system, isoforms CYP707A1, CYP707A3, CYP707A4 Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.137 cytochrome c 0.1 mM, complete inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.137 0.0013
-
 (+)-S-abscisate pH 7.25, 30°C, isoform CYP707A3 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.137 microsome
-
 Arabidopsis thaliana
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.137 52000
-
 x * 53000, isoform CYC707A1, x * 52000, isoform CYP707A3, SDS-PAGE Arabidopsis thaliana
1.14.14.137 53000
-
 x * 53000, isoform CYC707A1, x * 52000, isoform CYP707A3, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.137 Arabidopsis thaliana
-
 expression in baculovirus system, isoforms CYP707A1, CYP707A3, CYP707A4
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.137 (+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 isoform CYP707A3 is specific for (+)-isomer Arabidopsis thaliana 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O isoform CYP707A3, no hydroxylation at 7’ position ?
1.14.14.137 additional information isomerisation of 8’-hydroxy-abscisic acid to phaseic acid is not catalyzed by enzyme Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.137 ? x * 53000, isoform CYC707A1, x * 52000, isoform CYP707A3, SDS-PAGE Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.137 additional information NADH may not substitute for NADPH Arabidopsis thaliana