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Literature summary extracted from

  • Lee, M.; Martin, M.N.; Hudson, A.O.; Lee, J.; Muhitch, M.J.; Leustek, T.
    Methionine and threonine synthesis are limited by homoserine availability and not the activity of homoserine kinase in Arabidopsis thaliana (2005), Plant J., 41, 685-696.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.39 overexpressed in Arabidopsis thaliana Arabidopsis thaliana
4.2.3.1 expression in transgenic Arabidopsis Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.39 chloroplast soluble in stromal fraction Arabidopsis thaliana 9507
-
2.7.1.39 plastid
-
 Arabidopsis thaliana 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.39 K+ required Arabidopsis thaliana
2.7.1.39 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.39 ATP + L-homoserine Arabidopsis thaliana involved in the biosynthesis of threonine, isoleucine, and methionine, substrate availability rather than enzyme level rate limiting for the reaction ADP + O-phospho-L-homoserine
-
 ?
4.2.3.1 O-phospho-L-homoserine + H2O Escherichia coli threonine synthesis in eukaryotes L-threonine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.39 Arabidopsis thaliana Q8L7R2
-
-
4.2.3.1 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.1 O-phospho-L-homoserine + H2O = L-threonine + phosphate reaction proceeds via phosphate removal and isomerization from primary to secondary alcohol Escherichia coli
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.39 leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.1 additional information
-
 homozygous line from plant 829-2 exhibits 10.1fold and 2.1fold higher activity without and with 0.2 mmol/l S-adenosyl methionine compared to wild type. Homozygous line from plant 829-9 exhibits 25.2fold and 3.8fold higher activity without and with 0.2 mmol/l S-adenosyl methionine compared to wild type. Homozygous line from plant 829-14 exhibits 6.5fold and 1.5fold higher activity without and with 0.2 mmol/l S-adenosyl methionine compared to wild type Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.39 ATP + L-homoserine
-
 Arabidopsis thaliana ADP + O-phospho-L-homoserine
-
 ?
2.7.1.39 ATP + L-homoserine involved in the biosynthesis of threonine, isoleucine, and methionine, substrate availability rather than enzyme level rate limiting for the reaction Arabidopsis thaliana ADP + O-phospho-L-homoserine
-
 ?
4.2.3.1 O-phospho-L-homoserine + H2O threonine synthesis in eukaryotes Escherichia coli L-threonine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.1 threonine synthase
-
 Escherichia coli