Literature summary extracted from
Kleczkowski, L.A.; Martz, F.; Wilczynska, M.
Factors affecting oligomerization status of UDP-glucose pyrophosphorylase (2005), Phytochemistry, 66, 2815-2821.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.7.7.9 |
dithiothreitol |
increases wild-type activity by about 25% |
Hordeum vulgare |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.9 |
expression in Escherichia coli |
Hordeum vulgare |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.7.9 |
additional information |
not inhibitory: Tween-20 |
Hordeum vulgare |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.9 |
Hordeum vulgare |
Q43772 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.9 |
- |
Hordeum vulgare |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.7.7.9 |
additional information |
- |
similar activities of the wild-type UGPase in Tris, Hepes or Mops buffers. Half of the activity in 100 mM sodium phosphate. Compared with the wild-type UGPase the mutants KK127-128LL, C99S and LIV135-137NIN display reduced activities in all buffers. |
Hordeum vulgare |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.9 |
UTP + D-glucose-1-phosphate |
- |
Hordeum vulgare |
diphosphate + UDP-glucose |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.7.9 |
oligomer |
SDS-PAGE. Incubation of wild-type UGPase with phosphate or Tris buffers promote oligomerization, whereas Mops and Hepes completely dissociate the oligomers to monomers. Similar buffer effects for mutants KK127-128LL and C99S, small buffer effects for mutant LIV135-137NIN |
Hordeum vulgare |