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Literature summary extracted from

  • Van Rompay, A.R.; Johansson, M.; Karlsson, A.
    Substrate specificity and phosphorylation of antiviral and anticancer nucleoside analogues by human deoxyribonucleoside kinases and ribonucleoside kinases (2003), Pharmacol. Ther., 100, 119-139.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.74 medicine enzyme is active and important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview Homo sapiens
2.7.1.113 medicine enzyme is useful in anticancer therapy due to activation of anticancer drugs through phosphorylation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.74 genetic organization, located on chromosome 4q13.3-4q21.1, expression as GFP-fusion protein with location in the nucleus Homo sapiens
2.7.1.113 genetic organization, located on chromosome 2p13, expression as GFP-fusion protein in CHO cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 cytosol native enzyme Homo sapiens 5829
-
2.7.1.74 additional information the enzyme possesses a nuclear import signal sequence Homo sapiens
-
-
2.7.1.113 mitochondrial matrix
-
 Homo sapiens 5759
-
2.7.1.113 mitochondrion N-terminal enzyme sequence contains a mitochondrial import signal Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Mg2+
-
 Homo sapiens
2.7.1.113 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 ATP + 2'-deoxyadenosine Homo sapiens
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine Homo sapiens
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine Homo sapiens
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 additional information Homo sapiens de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview, enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview ?
-
 ?
2.7.1.74 UTP + 2'-deoxyadenosine Homo sapiens
-
 UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxycytidine Homo sapiens
-
 UDP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxyguanosine Homo sapiens
-
 UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyadenosine Homo sapiens
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyguanosine Homo sapiens
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyinosine Homo sapiens
-
 ADP + 2'-deoxyinosine 5'-phosphate
-
 ?
2.7.1.113 additional information Homo sapiens de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens P27707
-
-
2.7.1.113 Homo sapiens Q16854
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 bone marrow
-
 Homo sapiens
-
2.7.1.74 brain
-
 Homo sapiens
-
2.7.1.74 liver high enzyme content in fetal liver, very low content in adult liver Homo sapiens
-
2.7.1.74 additional information enzyme expression throughout the cell cycle, tissue-specific expression Homo sapiens
-
2.7.1.74 skeletal muscle
-
 Homo sapiens
-
2.7.1.74 thymus
-
 Homo sapiens
-
2.7.1.113 brain
-
 Homo sapiens
-
2.7.1.113 liver
-
 Homo sapiens
-
2.7.1.113 lymphoid tissue
-
 Homo sapiens
-
2.7.1.113 additional information enzyme is expressed in most tissues, enzyme expression throughout the cell cycle Homo sapiens
-
2.7.1.113 muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 additional information de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview, enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview Homo sapiens ?
-
 ?
2.7.1.74 additional information enzyme can utilizes several phosphate donors, with preference for UTP Homo sapiens ?
-
 ?
2.7.1.74 UTP + 1-beta-D-arabinofuranosylcytosine i.e. cytarabine Homo sapiens UDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2',2'-difluorodeoxycytidine i.e. gemcitabine Homo sapiens UDP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2',3'-dideoxycytidine
-
 Homo sapiens UDP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxy-3'-thiacytidine
-
 Homo sapiens UDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxyadenosine
-
 Homo sapiens UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxycytidine
-
 Homo sapiens UDP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxyguanosine
-
 Homo sapiens UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2-chloro-2'-deoxyadenosine i.e. cladribine Homo sapiens UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2-fluoro-9-beta-D-arabinofuranosyladenine i.e. fludarabine Homo sapiens UDP + 2-fluoro-9-beta-D-arabinofuranosyladenine 5'-phosphate
-
 ?
2.7.1.74 UTP + 9-beta-D-arabinofuranosylguanine i.e. nelarabine Homo sapiens UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyinosine
-
 Homo sapiens ADP + 2'-deoxyinosine 5'-phosphate
-
 ?
2.7.1.113 additional information de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview Homo sapiens ?
-
 ?
2.7.1.113 additional information anticancer drugs 2-chloro-2'-deoxyadenosine, i.e. cladribine, and 2-fluoro-9-beta-D-arabinofuranosyladenine, i.e. clofarabine, are poor substrates Homo sapiens ?
-
 ?
2.7.1.113 UTP + 2'-deoxyadenosine
-
 Homo sapiens UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.113 UTP + 2'-deoxyguanosine
-
 Homo sapiens UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.113 UTP + 2'-deoxyinosine
-
 Homo sapiens UDP + 2'-deoxyinosine 5'-phosphate
-
 ?
2.7.1.113 UTP + 9-beta-D-arabinofuranosylguanine i.e. nelarabine, activation of the anticancer drug Homo sapiens UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.74 dCK
-
 Homo sapiens
2.7.1.113 DGK
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.74 ATP
-
 Homo sapiens
2.7.1.74 UTP preferred, in vivo important phoaphate donor Homo sapiens
2.7.1.113 ATP
-
 Homo sapiens
2.7.1.113 additional information enzyme can use several phosphate donors, but UTP is preferred in vitro Homo sapiens
2.7.1.113 UTP preferred phosphate donor in vitro Homo sapiens