BRENDA - Enzyme Database show

Substrate specificity and phosphorylation of antiviral and anticancer nucleoside analogues by human deoxyribonucleoside kinases and ribonucleoside kinases

Van Rompay, A.R.; Johansson, M.; Karlsson, A.; Pharmacol. Ther. 100, 119-139 (2003)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.7.1.74
medicine
enzyme is active and important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
Homo sapiens
2.7.1.113
medicine
enzyme is useful in anticancer therapy due to activation of anticancer drugs through phosphorylation
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
genetic organization, located on chromosome 4q13.3-4q21.1, expression as GFP-fusion protein with location in the nucleus
Homo sapiens
2.7.1.113
genetic organization, located on chromosome 2p13, expression as GFP-fusion protein in CHO cells
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
native enzyme
Homo sapiens
5829
-
2.7.1.74
additional information
the enzyme possesses a nuclear import signal sequence
Homo sapiens
-
-
2.7.1.113
mitochondrial matrix
-
Homo sapiens
5759
-
2.7.1.113
mitochondrion
N-terminal enzyme sequence contains a mitochondrial import signal
Homo sapiens
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
additional information
Homo sapiens
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview, enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
?
-
-
-
2.7.1.74
UTP + 2'-deoxyadenosine
Homo sapiens
-
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
Homo sapiens
-
UDP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
Homo sapiens
-
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
Homo sapiens
-
ADP + 2'-deoxyinosine 5'-phosphate
-
-
?
2.7.1.113
additional information
Homo sapiens
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
P27707
-
-
2.7.1.113
Homo sapiens
Q16854
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
bone marrow
-
Homo sapiens
-
2.7.1.74
brain
-
Homo sapiens
-
2.7.1.74
liver
high enzyme content in fetal liver, very low content in adult liver
Homo sapiens
-
2.7.1.74
additional information
enzyme expression throughout the cell cycle, tissue-specific expression
Homo sapiens
-
2.7.1.74
skeletal muscle
-
Homo sapiens
-
2.7.1.74
thymus
-
Homo sapiens
-
2.7.1.113
brain
-
Homo sapiens
-
2.7.1.113
liver
-
Homo sapiens
-
2.7.1.113
lymphoid tissue
-
Homo sapiens
-
2.7.1.113
additional information
enzyme is expressed in most tissues, enzyme expression throughout the cell cycle
Homo sapiens
-
2.7.1.113
muscle
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxyadenosine
-
663023
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
663023
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
663023
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview, enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
663023
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
enzyme can utilizes several phosphate donors, with preference for UTP
663023
Homo sapiens
?
-
-
-
-
2.7.1.74
UTP + 1-beta-D-arabinofuranosylcytosine
i.e. cytarabine
663023
Homo sapiens
UDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2',2'-difluorodeoxycytidine
i.e. gemcitabine
663023
Homo sapiens
UDP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2',3'-dideoxycytidine
-
663023
Homo sapiens
UDP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxy-3'-thiacytidine
-
663023
Homo sapiens
UDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyadenosine
-
663023
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
-
663023
Homo sapiens
UDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
-
663023
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2-chloro-2'-deoxyadenosine
i.e. cladribine
663023
Homo sapiens
UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2-fluoro-9-beta-D-arabinofuranosyladenine
i.e. fludarabine
663023
Homo sapiens
UDP + 2-fluoro-9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 9-beta-D-arabinofuranosylguanine
i.e. nelarabine
663023
Homo sapiens
UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
-
663023
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
663023
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
-
663023
Homo sapiens
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
additional information
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview
663023
Homo sapiens
?
-
-
-
-
2.7.1.113
additional information
anticancer drugs 2-chloro-2'-deoxyadenosine, i.e. cladribine, and 2-fluoro-9-beta-D-arabinofuranosyladenine, i.e. clofarabine, are poor substrates
663023
Homo sapiens
?
-
-
-
-
2.7.1.113
UTP + 2'-deoxyadenosine
-
663023
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 2'-deoxyguanosine
-
663023
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 2'-deoxyinosine
-
663023
Homo sapiens
UDP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 9-beta-D-arabinofuranosylguanine
i.e. nelarabine, activation of the anticancer drug
663023
Homo sapiens
UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
UTP
preferred, in vivo important phoaphate donor
Homo sapiens
2.7.1.113
ATP
-
Homo sapiens
2.7.1.113
additional information
enzyme can use several phosphate donors, but UTP is preferred in vitro
Homo sapiens
2.7.1.113
UTP
preferred phosphate donor in vitro
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.1.74
medicine
enzyme is active and important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
Homo sapiens
2.7.1.113
medicine
enzyme is useful in anticancer therapy due to activation of anticancer drugs through phosphorylation
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
genetic organization, located on chromosome 4q13.3-4q21.1, expression as GFP-fusion protein with location in the nucleus
Homo sapiens
2.7.1.113
genetic organization, located on chromosome 2p13, expression as GFP-fusion protein in CHO cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
UTP
preferred, in vivo important phoaphate donor
Homo sapiens
2.7.1.113
ATP
-
Homo sapiens
2.7.1.113
additional information
enzyme can use several phosphate donors, but UTP is preferred in vitro
Homo sapiens
2.7.1.113
UTP
preferred phosphate donor in vitro
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
native enzyme
Homo sapiens
5829
-
2.7.1.74
additional information
the enzyme possesses a nuclear import signal sequence
Homo sapiens
-
-
2.7.1.113
mitochondrial matrix
-
Homo sapiens
5759
-
2.7.1.113
mitochondrion
N-terminal enzyme sequence contains a mitochondrial import signal
Homo sapiens
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
additional information
Homo sapiens
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview, enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
?
-
-
-
2.7.1.74
UTP + 2'-deoxyadenosine
Homo sapiens
-
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
Homo sapiens
-
UDP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
Homo sapiens
-
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
Homo sapiens
-
ADP + 2'-deoxyinosine 5'-phosphate
-
-
?
2.7.1.113
additional information
Homo sapiens
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
bone marrow
-
Homo sapiens
-
2.7.1.74
brain
-
Homo sapiens
-
2.7.1.74
liver
high enzyme content in fetal liver, very low content in adult liver
Homo sapiens
-
2.7.1.74
additional information
enzyme expression throughout the cell cycle, tissue-specific expression
Homo sapiens
-
2.7.1.74
skeletal muscle
-
Homo sapiens
-
2.7.1.74
thymus
-
Homo sapiens
-
2.7.1.113
brain
-
Homo sapiens
-
2.7.1.113
liver
-
Homo sapiens
-
2.7.1.113
lymphoid tissue
-
Homo sapiens
-
2.7.1.113
additional information
enzyme is expressed in most tissues, enzyme expression throughout the cell cycle
Homo sapiens
-
2.7.1.113
muscle
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxyadenosine
-
663023
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
663023
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
663023
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview, enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
663023
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
enzyme can utilizes several phosphate donors, with preference for UTP
663023
Homo sapiens
?
-
-
-
-
2.7.1.74
UTP + 1-beta-D-arabinofuranosylcytosine
i.e. cytarabine
663023
Homo sapiens
UDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2',2'-difluorodeoxycytidine
i.e. gemcitabine
663023
Homo sapiens
UDP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2',3'-dideoxycytidine
-
663023
Homo sapiens
UDP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxy-3'-thiacytidine
-
663023
Homo sapiens
UDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyadenosine
-
663023
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
-
663023
Homo sapiens
UDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
-
663023
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2-chloro-2'-deoxyadenosine
i.e. cladribine
663023
Homo sapiens
UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2-fluoro-9-beta-D-arabinofuranosyladenine
i.e. fludarabine
663023
Homo sapiens
UDP + 2-fluoro-9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 9-beta-D-arabinofuranosylguanine
i.e. nelarabine
663023
Homo sapiens
UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
-
663023
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
663023
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyinosine
-
663023
Homo sapiens
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
additional information
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview
663023
Homo sapiens
?
-
-
-
-
2.7.1.113
additional information
anticancer drugs 2-chloro-2'-deoxyadenosine, i.e. cladribine, and 2-fluoro-9-beta-D-arabinofuranosyladenine, i.e. clofarabine, are poor substrates
663023
Homo sapiens
?
-
-
-
-
2.7.1.113
UTP + 2'-deoxyadenosine
-
663023
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 2'-deoxyguanosine
-
663023
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 2'-deoxyinosine
-
663023
Homo sapiens
UDP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
UTP + 9-beta-D-arabinofuranosylguanine
i.e. nelarabine, activation of the anticancer drug
663023
Homo sapiens
UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?