Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takahashi-Abbe, S.; Abe, K.; Takahashi, N.
    Biochemical and functional properties of a pyruvate formate-lyase (PFL)-activating system in Streptococcus mutans (2003), Oral Microbiol. Immunol., 18, 293-297.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.54 additional information conversion into the active form is carried out by an activating system containing a flavodoxin system and an activating enzyme (pyruvate format lyase-activase, EC 1.97.1.4) Streptococcus mutans
2.3.1.54 NADPH more effective for activation than NADPH, 0.02 mM NADPH activates over 80% inactive enzyme in cell-free extracts Streptococcus mutans

Application

EC Number Application Comment Organism
1.97.1.4 medicine in dental plaque, under anaerobic conditions, enzyme is always kept active Streptococcus mutans

General Stability

EC Number General Stability Organism
2.3.1.54 stable for 2 h at 35°C in untreated cell-free extracts under strictly anaerobic conditions. In dialyzed cell-free extracts, activity decreases gradually. Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.97.1.4 40000
-
 gel filtration Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.4 Streptococcus mutans
-
-
-
2.3.1.54 Streptococcus mutans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.97.1.4
-
 Streptococcus mutans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.97.1.4 culture condition:anaerobically-grown cell
-
 Streptococcus mutans
-
2.3.1.54 culture condition:anaerobically-grown cell
-
 Streptococcus mutans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.97.1.4 105
-
 pH 6.8, 30°C Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
1.97.1.4 4°C, presence of Fe(SO4)2(NH4)2 and dithiothreitol, relatively stable Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.4 S-adenosyl-L-methionine + NADH + formate acetyltransferase-glycine
-
 Streptococcus mutans 5'-deoxyadenosine + methionine + NAD+ + formate acetyltransferase-glycine-2-yl-radical 40% of activity with reduced methyl viologen ?
1.97.1.4 S-adenosyl-L-methionine + NADPH + formate acetyltransferase-glycine 95% of activity with reduced methyl viologen Streptococcus mutans 5'-deoxyadenosine + methionine + NADP+ + formate acetyltransferase-glycine-2-yl-radical
-
 ?
1.97.1.4 S-adenosyl-L-methionine + reduced methyl viologen + formate acetyltransferase-glycine
-
 Streptococcus mutans 5'-deoxyadenosine + methionine + methyl viologen + formate acetyltransferase-glycine-2-yl-radical
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 pyruvate formate-lyase
-
 Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.97.1.4 6.8
-
-
 Streptococcus mutans
1.97.1.4 7.7
-
 20% of activity at pH 6.8 Streptococcus mutans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.97.1.4 Streptococcus mutans isoelectric focusing
-
 7.8