BRENDA - Enzyme Database show

Phosphorylation of isocarbostyril- and difluorophenyl-nucleoside thymidine mimics by the human deoxynucleoside kinases

Al-Madhoun, A.S.; Eriksson, S.; Wang, Z.X.; Naimi, E.; Knaus, E.E.; Wiebe, L.I.; Nucleosides Nucleotides Nucleic Acids 23, 1865-1874 (2004)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.001
-
2'-deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.0013
-
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.113
0.002
-
2'-deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.113
0.019
-
1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Homo sapiens
5829
-
2.7.1.113
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
2.7.1.113
Homo sapiens
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
additional information
-
catalytic efficiencies of the purified recombinant enzyme
Homo sapiens
2.7.1.113
additional information
-
catalytic efficiencies of the purified recombinant enzyme
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-isocarbostyril
poor substrate, 1.4% of the activity with 2'-deoxycytidine
662992
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
662992
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
nucleoside mimic, 14.6% of the activity with 2'-deoxycytidine
662992
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
additional information
no activity with several difluorophenyl nucleoside analogues and with 1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril, overview
662992
Homo sapiens
?
-
-
-
-
2.7.1.113
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
4.4% of the activity with 2'-deoxyguanosine
662992
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.113
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-isocarbostyril
poor substrate, 1.1% of the activity with 2'-deoxyguanosine
662992
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
662992
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
additional information
no activity with several difluorophenyl nucleoside analogues, overview
662992
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
2.7.1.113
37
-
assay at
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.02
-
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.11
-
2'-deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.113
0.007
-
1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.113
0.07
-
2'-deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7.6
-
assay at
Homo sapiens
2.7.1.113
7.6
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.113
ATP
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.113
ATP
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.001
-
2'-deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.0013
-
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.113
0.002
-
2'-deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.113
0.019
-
1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Homo sapiens
5829
-
2.7.1.113
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
additional information
-
catalytic efficiencies of the purified recombinant enzyme
Homo sapiens
2.7.1.113
additional information
-
catalytic efficiencies of the purified recombinant enzyme
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-isocarbostyril
poor substrate, 1.4% of the activity with 2'-deoxycytidine
662992
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
662992
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
nucleoside mimic, 14.6% of the activity with 2'-deoxycytidine
662992
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
additional information
no activity with several difluorophenyl nucleoside analogues and with 1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril, overview
662992
Homo sapiens
?
-
-
-
-
2.7.1.113
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
4.4% of the activity with 2'-deoxyguanosine
662992
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.113
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-isocarbostyril
poor substrate, 1.1% of the activity with 2'-deoxyguanosine
662992
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
662992
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
additional information
no activity with several difluorophenyl nucleoside analogues, overview
662992
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
2.7.1.113
37
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.02
-
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.11
-
2'-deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.113
0.007
-
1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.113
0.07
-
2'-deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7.6
-
assay at
Homo sapiens
2.7.1.113
7.6
-
assay at
Homo sapiens