BRENDA - Enzyme Database show

Immunocytochemical detection of deoxycytidine kinase in pediatric malignancies in relation to in vitro cytarabine sensitivity

Hubeek, I.; Peters, G.J.; Broekhuizen, A.J.F.; Talianidis, I.; Schouten van Meeteren, A.Y.N.; van Wering, E.R.; Gibson, B.; Creutzig, U.; Kaspers, G.J.L.; Nucleosides Nucleotides Nucleic Acids 23, 1351-1356 (2004)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + cytarabine
Homo sapiens
drug against acute myeloid and lymphoblastic leukemia
ADP + cytarabine 5'-phosphate
-
-
?
2.7.1.74
additional information
Homo sapiens
resistance of cells to cytarabine is caused by dCK enzyme deficiency
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
brain tumor cell
low enzyme expression level
Homo sapiens
-
2.7.1.74
additional information
high enzyme expression level in case of acute myeloid leukemia and acute lymphoblastic leukemia, sensitivity of cell types to cytarabine, overview
Homo sapiens
-
2.7.1.74
retinoblastoma cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
additional information
-
MTT assay method
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
662989
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + cytarabine
drug against acute myeloid and lymphoblastic leukemia
662989
Homo sapiens
ADP + cytarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + cytarabine
i.e. 1-beta-D-arabinofuranosylcytosine
662989
Homo sapiens
ADP + cytarabine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
resistance of cells to cytarabine is caused by dCK enzyme deficiency
662989
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + cytarabine
Homo sapiens
drug against acute myeloid and lymphoblastic leukemia
ADP + cytarabine 5'-phosphate
-
-
?
2.7.1.74
additional information
Homo sapiens
resistance of cells to cytarabine is caused by dCK enzyme deficiency
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
brain tumor cell
low enzyme expression level
Homo sapiens
-
2.7.1.74
additional information
high enzyme expression level in case of acute myeloid leukemia and acute lymphoblastic leukemia, sensitivity of cell types to cytarabine, overview
Homo sapiens
-
2.7.1.74
retinoblastoma cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
additional information
-
MTT assay method
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
662989
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + cytarabine
drug against acute myeloid and lymphoblastic leukemia
662989
Homo sapiens
ADP + cytarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + cytarabine
i.e. 1-beta-D-arabinofuranosylcytosine
662989
Homo sapiens
ADP + cytarabine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
resistance of cells to cytarabine is caused by dCK enzyme deficiency
662989
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens