BRENDA - Enzyme Database show

Fluorescence studies of substrate binding to human recombinant deoxycytidine kinase

Mani, R.S.; Usova, E.V.; Eriksson, S.; Cass, C.E.; Nucleosides Nucleotides Nucleic Acids 23, 1343-1346 (2004)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCTP
feedback inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
steady-state fluorescent measurements, substrate binding constants, recombinant enzyme
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
additional information
Homo sapiens
enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
?
-
-
-
2.7.1.74
UTP + 2'-deoxyadenosine
Homo sapiens
-
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
Homo sapiens
preferred donor and acceptor substrates
UDP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
Homo sapiens
-
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxyadenosine
-
662988
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
662988
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
662988
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + adenosine
-
662988
Homo sapiens
ADP + adenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + arabinosyl cytosine
i.e. cytosar
662988
Homo sapiens
ADP + arabinosyl cytosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + cladribine
-
662988
Homo sapiens
ADP + cladribine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + cytidine
-
662988
Homo sapiens
ADP + cytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + fludarabine
-
662988
Homo sapiens
ADP + fludarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + gemcitabine
-
662988
Homo sapiens
ADP + gemcitabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + L-2',3'-dideoxy-3'-thiacytidine
-
662988
Homo sapiens
ADP + L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
662988
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
broad substrate specificity, overview, binding of acceptor subtrates is more tightly in presence of phosphate donor molecules
662988
Homo sapiens
?
-
-
-
-
2.7.1.74
UTP + 2'-deoxyadenosine
-
662988
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
preferred donor and acceptor substrates
662988
Homo sapiens
UDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
-
662988
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + adenosine
-
662988
Homo sapiens
UDP + adenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + arabinosyl cytosine
i.e. cytosar
662988
Homo sapiens
UDP + arabinosyl cytosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + cladribine
-
662988
Homo sapiens
UDP + cladribine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + cytidine
-
662988
Homo sapiens
UDP + cytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + fludarabine
-
662988
Homo sapiens
UDP + fludarabine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + gemcitabine
-
662988
Homo sapiens
UDP + gemcitabine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + L-2',3'-dideoxy-3'-thiacytidine
-
662988
Homo sapiens
UDP + L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
UTP
preferred phosphate donor
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
steady-state fluorescent measurement, inhibitor binding constant, recombinant enzyme
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
UTP
preferred phosphate donor
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCTP
feedback inhibition
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
steady-state fluorescent measurement, inhibitor binding constant, recombinant enzyme
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
steady-state fluorescent measurements, substrate binding constants, recombinant enzyme
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.74
additional information
Homo sapiens
enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
?
-
-
-
2.7.1.74
UTP + 2'-deoxyadenosine
Homo sapiens
-
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
Homo sapiens
preferred donor and acceptor substrates
UDP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
Homo sapiens
-
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxyadenosine
-
662988
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
662988
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
662988
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + adenosine
-
662988
Homo sapiens
ADP + adenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + arabinosyl cytosine
i.e. cytosar
662988
Homo sapiens
ADP + arabinosyl cytosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + cladribine
-
662988
Homo sapiens
ADP + cladribine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + cytidine
-
662988
Homo sapiens
ADP + cytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + fludarabine
-
662988
Homo sapiens
ADP + fludarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + gemcitabine
-
662988
Homo sapiens
ADP + gemcitabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + L-2',3'-dideoxy-3'-thiacytidine
-
662988
Homo sapiens
ADP + L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
662988
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
broad substrate specificity, overview, binding of acceptor subtrates is more tightly in presence of phosphate donor molecules
662988
Homo sapiens
?
-
-
-
-
2.7.1.74
UTP + 2'-deoxyadenosine
-
662988
Homo sapiens
UDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
preferred donor and acceptor substrates
662988
Homo sapiens
UDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + 2'-deoxyguanosine
-
662988
Homo sapiens
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + adenosine
-
662988
Homo sapiens
UDP + adenosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + arabinosyl cytosine
i.e. cytosar
662988
Homo sapiens
UDP + arabinosyl cytosine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + cladribine
-
662988
Homo sapiens
UDP + cladribine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + cytidine
-
662988
Homo sapiens
UDP + cytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + fludarabine
-
662988
Homo sapiens
UDP + fludarabine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + gemcitabine
-
662988
Homo sapiens
UDP + gemcitabine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + L-2',3'-dideoxy-3'-thiacytidine
-
662988
Homo sapiens
UDP + L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?