BRENDA - Enzyme Database show

Mosquito has a single multisubstrate deoxyribonucleoside kinase characterized by unique substrate specificity

Knecht, W.; Petersen, G.E.; Sandrini, M.P.; Sondergaard, L.; Munch-Petersen, B.; Piskur, J.; Nucleic Acids Res. 31, 1665-1672 (2003)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.7.1.145
medicine
enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer
Anopheles gambiae
2.7.1.145
synthesis
enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes
Anopheles gambiae
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.145
DNA and amino acid sequence determination and analysis, overexpression in TK-deficient Escherichia coli strain KY895 as GST-fusion protein
Anopheles gambiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.0058
-
2'-deoxythymidine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.0095
-
2'-deoxyguanosine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.0126
-
2'-deoxycytidine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.0181
-
2'-deoxyadenosine
pH 8.0, recombinant enzyme
Anopheles gambiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.145
Mg2+
-
Anopheles gambiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.145
ATP + 2'-deoxyadenosine
Anopheles gambiae
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
Anopheles gambiae
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
Anopheles gambiae
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
Anopheles gambiae
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Anopheles gambiae
Q86LB8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.145
native enzyme from cell lines in a 5-step procedure, recombinant GST-fusion enzyme from Escherichia coli strain KY895 by GST-affinity chromatography
Anopheles gambiae
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.145
4a-2s4 cell
-
Anopheles gambiae
-
2.7.1.145
L3-5-3 cell
-
Anopheles gambiae
-
2.7.1.145
Sua1.1 cell
-
Anopheles gambiae
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
additional information
-
substrate specificities of cell lines
Anopheles gambiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
high activity
662967
Anopheles gambiae
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
low activity
662967
Anopheles gambiae
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylthymine
low activity
662967
Anopheles gambiae
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-didehydro-3'-deoxythymidine
low activity
662967
Anopheles gambiae
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
662967
Anopheles gambiae
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
662967
Anopheles gambiae
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
high activity
662967
Anopheles gambiae
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
662967
Anopheles gambiae
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
high activity
662967
Anopheles gambiae
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
662967
Anopheles gambiae
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2-chloro-2'-deoxyadenosine
best substrate
662967
Anopheles gambiae
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 5-fluoro-2'-deoxyuridine
-
662967
Anopheles gambiae
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + stavudine
anti-HIV nucleoside analogue
662967
Anopheles gambiae
ADP + stavudine phosphate
-
-
-
?
2.7.1.145
additional information
substrate specificity, no activity with cytidine, adenine, guanine, and thymine, no activity with dideoxynucleosides, and with 3'-azido-2',3'-didehydro-3'-deoxythymidine
662967
Anopheles gambiae
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.014
-
2'-deoxythymidine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.019
-
2'-deoxyguanosine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.021
-
2'-deoxyadenosine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.03
-
2'-deoxycytidine
pH 8.0, recombinant enzyme
Anopheles gambiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.145
8
-
assay at
Anopheles gambiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.145
ATP
-
Anopheles gambiae
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.1.145
medicine
enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer
Anopheles gambiae
2.7.1.145
synthesis
enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes
Anopheles gambiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
DNA and amino acid sequence determination and analysis, overexpression in TK-deficient Escherichia coli strain KY895 as GST-fusion protein
Anopheles gambiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.145
ATP
-
Anopheles gambiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.0058
-
2'-deoxythymidine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.0095
-
2'-deoxyguanosine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.0126
-
2'-deoxycytidine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.0181
-
2'-deoxyadenosine
pH 8.0, recombinant enzyme
Anopheles gambiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.145
Mg2+
-
Anopheles gambiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.145
ATP + 2'-deoxyadenosine
Anopheles gambiae
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
Anopheles gambiae
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
Anopheles gambiae
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
Anopheles gambiae
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
native enzyme from cell lines in a 5-step procedure, recombinant GST-fusion enzyme from Escherichia coli strain KY895 by GST-affinity chromatography
Anopheles gambiae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.145
4a-2s4 cell
-
Anopheles gambiae
-
2.7.1.145
L3-5-3 cell
-
Anopheles gambiae
-
2.7.1.145
Sua1.1 cell
-
Anopheles gambiae
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
additional information
-
substrate specificities of cell lines
Anopheles gambiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
high activity
662967
Anopheles gambiae
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
low activity
662967
Anopheles gambiae
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylthymine
low activity
662967
Anopheles gambiae
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-didehydro-3'-deoxythymidine
low activity
662967
Anopheles gambiae
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
662967
Anopheles gambiae
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
662967
Anopheles gambiae
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
high activity
662967
Anopheles gambiae
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
662967
Anopheles gambiae
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
high activity
662967
Anopheles gambiae
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
662967
Anopheles gambiae
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2-chloro-2'-deoxyadenosine
best substrate
662967
Anopheles gambiae
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 5-fluoro-2'-deoxyuridine
-
662967
Anopheles gambiae
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + stavudine
anti-HIV nucleoside analogue
662967
Anopheles gambiae
ADP + stavudine phosphate
-
-
-
?
2.7.1.145
additional information
substrate specificity, no activity with cytidine, adenine, guanine, and thymine, no activity with dideoxynucleosides, and with 3'-azido-2',3'-didehydro-3'-deoxythymidine
662967
Anopheles gambiae
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.014
-
2'-deoxythymidine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.019
-
2'-deoxyguanosine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.021
-
2'-deoxyadenosine
pH 8.0, recombinant enzyme
Anopheles gambiae
2.7.1.145
0.03
-
2'-deoxycytidine
pH 8.0, recombinant enzyme
Anopheles gambiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.145
8
-
assay at
Anopheles gambiae