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Literature summary extracted from

  • Johansson, K.; Ramaswamy, S.; Ljungcrantz, C.; Knecht, W.; Piskur, J.; Munch-Petersen, B.; Eriksson, S.; Eklund, H.
    Structural basis for substrate specificities of cellular deoxyribonucleoside kinases (2001), Nat. Struct. Biol., 8, 616-620.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.113 medicine the enzyme activates a number of medically important nucleoside analogues Homo sapiens
2.7.1.145 medicine the enzyme activates a number of medically important nucleoside analogues Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.113 purified recombinant enzyme, hanging drop vapour diffusion method, enzyme solution containing 7 mg/ml protein, 5 mM MgCl2, and 5 mM ATP, mixed with an equal volume of cyrstallization solution containing 0.1 M Na-citrate, pH 6.5, 0.2 M ammonium acetate, and 30% w/v PEG 4000, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement method Homo sapiens
2.7.1.145 purified recombinant enzyme, hanging drop vapour diffusion method, enzyme solution containing 10 mg/ml protein and 10 mM deoxycytidine, mixed with an equal volume of cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 20% w/v PEG 5000, and 8-10% v/v PEG 400, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement method Drosophila melanogaster

General Stability

EC Number General Stability Organism
2.7.1.145 ATP stabilizes the enzyme structure Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.113 Mg2+
-
 Homo sapiens
2.7.1.145 Mg2+
-
 Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.113 Homo sapiens Q16854
-
-
2.7.1.145 Drosophila melanogaster Q9XZT6
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.113 ATP + deoxyguanosine = ADP + dGMP substrate interactions and binding mechanism, and active site structure Homo sapiens
a
2.7.1.145 ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate substrate binding and interactions Drosophila melanogaster
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.113 ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.113 additional information the enzyme is highly specific for purine substrates, Arg118 is responsible for the substrate specificity Homo sapiens ?
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 additional information the enzyme shows broad substrate specificity probably due to a wide substrate binding cleft Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.113 DGK
-
 Homo sapiens
2.7.1.145 dNK
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.113 ATP
-
 Homo sapiens
2.7.1.145 ATP stabilizes the enzyme structure, binding structure, Q111 and Arg118 are involved Drosophila melanogaster