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Literature summary extracted from

  • Gross, S.; Piwnica-Worms, D.
    Real-time imaging of ligand-induced IKK activation in intact cells and in living mice (2005), Nat. Methods, 2, 607-614.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.11.10 analysis development of real-time imaging for continous enzyme detection and kinetics in intact cells and living mice utilizing a recombinant IkappaBalpha-firefly luciferase reporter construct, system can be used for determination of kinetics/pharmacodynamics of potential selective inhibitors, and for investigations of NF-kappaB signalling pathway activation Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.10 ATP + [IkappaB protein] Mus musculus phosphorylation of IkappaB results in its proteolytic degradation ADP + [IkappaB phosphoprotein]
-
 ?
2.7.11.10 additional information Mus musculus IKK is required for activation of NF-kappaB and subsequent signalling pathways ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.10 Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.10 additional information
-
 development of real-time imaging for continous enzyme detection and kinetics in intact cells and living mice utilizing a recombinant IkappaBalpha-firefly luciferase reporter construct Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.10 ATP + [IkappaB protein]
-
 Mus musculus ADP + [IkappaB phosphoprotein]
-
 ?
2.7.11.10 ATP + [IkappaB protein] phosphorylation of IkappaB results in its proteolytic degradation Mus musculus ADP + [IkappaB phosphoprotein]
-
 ?
2.7.11.10 additional information IKK is required for activation of NF-kappaB and subsequent signalling pathways Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.10 IKK
-
 Mus musculus