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Literature summary extracted from

  • Skladanowski, A.; Come, M.G.; Sabisz, M.; Escargueil, A.E.; Larsen, A.K.
    Down-regulation of DNA topoisomerase IIalpha leads to prolonged cell cycle transit in G2 and early M phases and increased survival to microtubule-interacting agents (2005), Mol. Pharmacol., 68, 625-634.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.2 expressed in DC-3F cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 amsacrine reduced inhibition of the enzyme in vincristine-resistant cells Homo sapiens
5.6.2.2 ICRF-187 reduced inhibition of the enzyme in vincristine-resistant cells Homo sapiens
5.6.2.2 Mitoxantrone reduced inhibition of the enzyme in vincristine-resistant cells Homo sapiens
5.6.2.2 additional information vincristine selection and resistance is accompanied by specific downregulation of isozyme IIalpha, but of isozyme IIbeta, leading to at least 7fold reduced chromosome-associated isozyme IIalpha Homo sapiens
5.6.2.2 Symadex i.e. C-1311, reduced inhibition of the enzyme in vincristine-resistant cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 KCl required for decatenation activity Homo sapiens
5.6.2.2 Mg2+ required for decatenation activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.2.2 additional information Homo sapiens the amount of isozyme IIalpha in cells influences directly the cell cycle kinetics in G2 and early mitosis as well as the resistance to nocodazole ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Homo sapiens
-
 isozymes IIalpha and IIbeta
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 HL-60 cell vincristine-resistant leukemia cell line Homo sapiens
-
5.6.2.2 HL-60/Vinc cell vincristine-selected multidrug-resistant HL-60 cells Homo sapiens
-
5.6.2.2 additional information isozyme IIalpha is important in cell cycle kinetics in G2 and early M phases Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 dsDNA + ATP + H2O decatenation of dsDNA Homo sapiens ?
-
 ?
5.6.2.2 additional information the amount of isozyme IIalpha in cells influences directly the cell cycle kinetics in G2 and early mitosis as well as the resistance to nocodazole Homo sapiens ?
-
 ?
5.6.2.2 additional information isozyme IIalpha interacts with the microtubule-directed agent nocodazole, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 DNA topoisomerase II
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.2 37
-
 decatenation assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.2 7.5
-
 decatenation assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.2 ATP required for decatenation activity Homo sapiens