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Literature summary extracted from

  • Ullan, R.V.; Casqueiro, J.; Naranjo, L.; Vaca, I.; Martin, J.F.
    Expression of cefD2 and the conversion of isopenicillin N into penicillin N by the two-component epimerase system are rate-limiting steps in cephalosporin biosynthesis (2004), Mol. Genet. Genomics, 272, 562-570.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.1.17 synthesis epimerization is a rate-limiting step in cephalosporin biosynthesis in Acremonium chrysogenum. When epimerase activity is enhanced, there is a clear increase in cephalosporin production. Thus, this method provides a strategy that could easily be applied to improve industrial cephalosporin producing strains Acremonium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.17 isopenicillin N Acremonium chrysogenum
-
 penicillin N
-
 ?
5.1.1.17 isopenicillin N Acremonium chrysogenum C10 / ATCC 48272
-
 penicillin N
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.17 Acremonium chrysogenum
-
 and transformant strains TMCD2, TMCD26, TMCD32, TMCD39, TMCD53, TMCD242, TMCD474
-
5.1.1.17 Acremonium chrysogenum C10 / ATCC 48272
-
 and transformant strains TMCD2, TMCD26, TMCD32, TMCD39, TMCD53, TMCD242, TMCD474
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.1.17 mycelium
-
 Acremonium chrysogenum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.17 additional information
-
 strain C10 (ATCC 48272): 93 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD2: no activity, transformant strain TMCD26: 223 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD32: no activity, transformant strain TMCD39: no activity, transformant strain TMCD53: 226 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD242: 234 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD474: 202 ng of cephalosporin C formed per min and mg of protein Acremonium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.17 isopenicillin N
-
 Acremonium chrysogenum penicillin N
-
 ?
5.1.1.17 isopenicillin N
-
 Acremonium chrysogenum C10 / ATCC 48272 penicillin N
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.1.17 IPN epimerase
-
 Acremonium chrysogenum
5.1.1.17 isopenicillin N-CoA epimerase
-
 Acremonium chrysogenum