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Literature summary extracted from

  • Kim, D.W.; Yoon, C.S.; Eum, W.S.; Lee, B.R.; An, J.J.; Lee, S.H.; Lee, S.R.; Ahn, J.Y.; Kwon, O.S.; Kang, T.C.; Won, M.H.; Cho, S.W.; Lee, K.S.; Park, J.; Choi, S.Y.
    Site-directed mutagenesis of human brain GABA transaminase: lysine-357 is involved in cofactor binding at the active site (2004), Mol. Cells, 18, 314-319.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.19 K357A no enzymic activity, even not by addition of exogenous pyridoxal 5’-phosphate Homo sapiens
2.6.1.19 K357B no enzymic activity, even not by addition of exogenous pyridoxal 5’-phosphate Homo sapiens
2.6.1.19 K357N no enzymic activity, even not by addition of exogenous pyridoxal 5’-phosphate Homo sapiens
2.6.1.19 K357Q no enzymic activity, even not by addition of exogenous pyridoxal 5’-phosphate Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.19 50000
-
 x * 50000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.19 Homo sapiens
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.6.1.19 apoenzyme reconstituted with exogenous pyridoxal 5’-phosphate almost completely regains catalytic activity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.19 brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.19 17.5
-
 pH 8.4, 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.19 4-aminobutanoate + 2-oxoglutarate
-
 Homo sapiens 4-oxobutanoate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.19 ? x * 50000, SDS-PAGE Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.19 pyridoxal 5'-phosphate K357 is involved in binding Homo sapiens