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Literature summary extracted from

  • Field, J.; Wilson, M.P.; Mai, Z.; Majerus, P.W.; Samuelson, J.
    An Entamoeba histolytica inositol 1,3,4-trisphosphate 5/6-kinase has a novel 3-kinase activity (2000), Mol. Biochem. Parasitol., 108, 119-123.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.159 additional information enzyme expression is slightly induced by heat shock Entamoeba histolytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.159 DNA and amino acid sequence determination and analysis, expression as GST-tagged enzyme in bacteria Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate Entamoeba histolytica
-
 ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate Entamoeba histolytica
-
 ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate Entamoeba histolytica HM-1
-
 ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate Entamoeba histolytica HM-1
-
 ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.159 Entamoeba histolytica Q9XYQ1
-
-
2.7.1.159 Entamoeba histolytica HM-1 Q9XYQ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.159 recombinant GST-tagged enzyme, the GST-tag is cleaved off by treatment with GST-3Cpro Entamoeba histolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.159 additional information
-
-
 Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate the formation of 1D-myo-inositol-1,3,4,6-tetrakisphosphate is slightly preferred compared to formation of 1D-myo-inositol-1,3,4,5-tetrakisphosphate, ratio 1.5:1 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica HM-1 ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica HM-1 ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate the formation of 1D-myo-inositol-1,3,4,6-tetrakisphosphate is slightly preferred compared to formation of 1D-myo-inositol-1,3,4,5-tetrakisphosphate, ratio 1.5:1 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate the formation of 1D-myo-inositol-1,3,4,6-tetrakisphosphate is slightly preferred compared to formation of 1D-myo-inositol-1,3,4,5-tetrakisphosphate, ratio 1.5:1 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica HM-1 ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica HM-1 ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate the formation of 1D-myo-inositol-1,3,4,6-tetrakisphosphate is slightly preferred compared to formation of 1D-myo-inositol-1,3,4,5-tetrakisphosphate, ratio 1.5:1 ?
2.7.1.159 additional information the enzyme also preferably performs the phosphoinositol trisphosphate 3-kinase reaction with 1D-myo-inositol-1,4,5-trisphosphate as substrate, EC 2.7.1.127, no activity with 1D-myo-inositol-1,4-bisphosphate Entamoeba histolytica ?
-
 ?
2.7.1.159 additional information the enzyme also preferably performs the phosphoinositol trisphosphate 3-kinase reaction with 1D-myo-inositol-1,4,5-trisphosphate as substrate, EC 2.7.1.127, no activity with 1D-myo-inositol-1,4-bisphosphate Entamoeba histolytica HM-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.159 Ins(1,3,4)P3 5/6-kinase
-
 Entamoeba histolytica

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.159 ATP
-
 Entamoeba histolytica