Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Krehenbrink, M.; Steinbüchel, A.
    Partial purification and characterization of a non-cyanobacterial cyanophycin synthetase from Acinetobacter calcoaceticus strain ADP1 with regard to substrate specificity, substrate affinity and binding to cyanophycin (2004), Microbiology, 150, 2599-2608.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.29 expression in Escherichia coli DH1 cells Acinetobacter calcoaceticus
6.3.2.30 expression in Escherichia coli DH1 cells Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.29 0.038
-
 ATP 30°C, pH 8.2, for the L-arginine-ATP binding site alone Acinetobacter calcoaceticus
6.3.2.29 0.047
-
 L-arginine 30°C, pH 8.2 Acinetobacter calcoaceticus
6.3.2.29 0.278
-
 ATP 30°C, pH 8.2, for the complete reaction Acinetobacter calcoaceticus
6.3.2.30 0.21
-
 ATP 30°C, pH 8.2, for the L-aspartic acid-ATP binding site alone Acinetobacter calcoaceticus
6.3.2.30 0.24
-
 L-aspartic acid 30°C, pH 8.2 Acinetobacter calcoaceticus
6.3.2.30 0.278
-
 ATP 30°C, pH 8.2, for the complete reaction Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.29 Mg2+
-
 Acinetobacter calcoaceticus
6.3.2.30 Mg2+
-
 Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp Acinetobacter calcoaceticus
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 additional information Acinetobacter calcoaceticus without L-arginine 1.1% activity compared to the reaction mixture containing both substrates, no activity using L-lysine instead of L-arginine, no activity without addition of small amounts of cyanophycin as a primer for synthesis ?
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Acinetobacter calcoaceticus
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 additional information Acinetobacter calcoaceticus without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.29 Acinetobacter calcoaceticus
-
-
-
6.3.2.30 Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.29 anion-exchange chromatography, gel filtration, affinity precipitation with cyanophycin, 69fold purification Acinetobacter calcoaceticus
6.3.2.30 anion-exchange chromatography, gel filtration, affinity precipitation with cyanophycin, 69fold purification Acinetobacter calcoaceticus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp = ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp i.e. the second reaction of cyanophycin synthesis catalysed by cyanophycin synthase, the first reaction is catalysed by the enzyme's other active centre Acinetobacter calcoaceticus
a
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the enzyme's other active centre Acinetobacter calcoaceticus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.29 0.265
-
 30°C, pH 8.2 Acinetobacter calcoaceticus
6.3.2.30 0.265
-
 30°C, pH 8.2 Acinetobacter calcoaceticus

Storage Stability

EC Number Storage Stability Organism
6.3.2.29 at 7°C the purified enzyme is stable for about one week, thereafter the activity decreases rapidly, storage at -20°C in the presence of 10% (w/v) DMSO or 50% (w/v) glycerol does not improve the stability significantly Acinetobacter calcoaceticus
6.3.2.30 at 7°C the purified enzyme is stable for about one week, thereafter the activity decreases rapidly, storage at -20°C in the presence of 10% (w/v) DMSO or 50% (w/v) glycerol does not improve the stability significantly Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.29 ATP + cyanophycin granule polypeptide-L-Asp + L-arginine
-
 Acinetobacter calcoaceticus ?
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 Acinetobacter calcoaceticus ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 additional information without L-arginine 1.1% activity compared to the reaction mixture containing both substrates, no activity using L-lysine instead of L-arginine, no activity without addition of small amounts of cyanophycin as a primer for synthesis Acinetobacter calcoaceticus ?
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Acinetobacter calcoaceticus ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 L-aspartic acid + ATP
-
 Acinetobacter calcoaceticus poly-L-aspartic acid + ADP + phosphate
-
 ?
6.3.2.30 additional information without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis Acinetobacter calcoaceticus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.29 cyanophycin synthetase
-
 Acinetobacter calcoaceticus
6.3.2.30 cyanophycin synthetase
-
 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.29 8.5
-
 30°C Acinetobacter calcoaceticus
6.3.2.30 8.5
-
 30°C Acinetobacter calcoaceticus