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Literature summary extracted from

  • Louis, P.; Galinski, E.A.
    Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in Escherichia coli (1997), Microbiology, 143, 1141-1149.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.108 additional information high salinity/osmolarity of the medium induces enzyme expression Marinococcus halophilus

Application

EC Number Application Comment Organism
4.2.1.108 biotechnology inducible recombinant enzyme expression in Escherichia coli confers the capability to grow on high concentration of osmolytes by increasing the osmotolerance via L-ectoine production Marinococcus halophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.178 expression in Escherichia coli, also osmoregulated in Escherichia coli Marinococcus halophilus
4.2.1.108 gene ectC, DNA and amino acid sequence determination and analysis, genetic mapping and organization, functional expression in Escherichia coli, the recombinant enzyme expression is osmoregulated in response to medium salinity in Escherichia coli Marinococcus halophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Marinococcus halophilus final step in L-ectoine biosynthesis, pathway overview L-ectoine + H2O
-
 ?
4.2.1.108 additional information Marinococcus halophilus the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.178 Marinococcus halophilus
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-
-
4.2.1.108 Marinococcus halophilus O06061 gene ectC
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview Marinococcus halophilus L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Marinococcus halophilus L-ectoine + H2O
-
 ?
4.2.1.108 additional information the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes Marinococcus halophilus ?
-
 ?