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Literature summary extracted from

  • Bhuiyan, S.H.; Kitaoka, M.; Hayashi, K.
    A cycloamylose-forming hyperthermostable 4-alpha-glucanotransferase of Aquifex aeolicus expressed in Escherichia coli (2003), J. Mol. Catal. B, 22, 45-53.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 4.2
-
 maltopentaose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 4.5
-
 maltotetraose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 4.5
-
 maltohexaose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 5.7
-
 maltoheptaose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 6.1
-
 maltotriose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 71
-
 maltose pH 6.6, 70°C Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Aquifex aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
 Aquifex aeolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.25 44.2
-
-
 Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 amylose the enzyme produces a cycloamylose with a minimum degree of polymerization of 16 Aquifex aeolicus cycloamylose
-
 ?
2.4.1.25 maltoheptaose + maltoheptaose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
2.4.1.25 maltohexaose + maltohexaose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
2.4.1.25 maltose + maltose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
2.4.1.25 maltotetraose + maltotetraose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
2.4.1.25 maltotriose + maltotriose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
2.4.1.25 additional information the smallest acceptor is glucose, the smallest donor is maltose Aquifex aeolicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 4-alpha-glucanotransferase
-
 Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 90
-
-
 Aquifex aeolicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.25 60 100 60°C: about 50% of maximal activity, 100°C: about 30% of maximal activity Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 80
-
 30 min, less than 10% loss of activity Aquifex aeolicus
2.4.1.25 90
-
 30 min, about 30% loss of activity Aquifex aeolicus
2.4.1.25 100
-
 30 min, about 90% loss of activity Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.25 1.3
-
 maltose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 16
-
 maltoheptaose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 17
-
 maltotetraose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 18
-
 maltotriose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 22
-
 maltopentaose pH 6.6, 70°C Aquifex aeolicus
2.4.1.25 22
-
 maltohexaose pH 6.6, 70°C Aquifex aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6.5
-
-
 Aquifex aeolicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.25 3 11 stable Aquifex aeolicus