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Literature summary extracted from

  • Hible, G.; Renault, L.; Schaeffer, F.; Christova, P.; Zoe Radulescu, A.; Evrin, C.; Gilles, A.M.; Cherfils, J.
    Calorimetric and crystallographic analysis of the oligomeric structure of Escherichia coli GMP kinase (2005), J. Mol. Biol., 352, 1044-1059.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.8 expression in Escherichia coli strain BLI5 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.8
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.8 23460
-
 monomer, electrospray ionization-mass spectrometry Escherichia coli
2.7.4.8 23462
-
 x * 23462, thermodynamic analysis, the enzyme is in equilibrium between a dimer and higher order oligomers, whose relative amounts depend on protein concentration, ionic strength, and the presence of ATP Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.8 ATP + dGMP Escherichia coli
-
 ADP + dGDP
-
 r
2.7.4.8 ATP + GMP Escherichia coli
-
 ADP + GDP
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Escherichia coli P60546
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 recombinant ecGMPK is purified by a two-step chromatography procedure involving affinity chromatography on Blue Sepharose and gel filtration Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.4.8 complete denaturation of the protein is not reversible due to aggregation at the unfolded state Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.8 -20°C, stable for several months after purification by Blue Sepharose and gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + dGMP
-
 Escherichia coli ADP + dGDP
-
 r
2.7.4.8 ATP + GMP
-
 Escherichia coli ADP + GDP
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.8 hexamer crystallography Escherichia coli
2.7.4.8 oligomer x * 23462, thermodynamic analysis, the enzyme is in equilibrium between a dimer and higher order oligomers, whose relative amounts depend on protein concentration, ionic strength, and the presence of ATP Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.4.8 ecGMPK
-
 Escherichia coli
2.7.4.8 GMP kinase
-
 Escherichia coli
2.7.4.8 GMPK
-
 Escherichia coli