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Literature summary extracted from

  • Yang, J.; Ten Eyck, L.F.; Xuong, N.H.; Taylor, S.S.
    Crystal structure of a cAMP-dependent protein kinase mutant at 1.26A: new insights into the catalytic mechanism (2004), J. Mol. Biol., 336, 473-487.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.11 expression of wild-type and mutant Y204A in Escherichia coli Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.11.11 purified recombinant wild-type and mutant PKA catalytic subunits, 0.002 ml 5 mg/ml protein in 50 mM bicine, pH 8.0, 200 mM ammonium acetate, 2 mM DTT, MgCl2, ATP, and IP20, is mixed with 0.001 ml well solution containing 8-12% v/v 2-methyl-2,4-pentanediol and 10 mM DTT, addition of 7% v/v methanol before sealing, X-ray diffraction structure determination and analysis at 1.26 A resolution, modeling Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.11 Y204A site-directed mutagenesis, mutation in the catalytic subunit, reduced catalytic efficiency compared to the wild-type enzyme Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 IP20 inhibitory peptide TTYADFIASGRTGRRN, residues 5-24 of inhibitor PKI, inhibits the ATPase function of the enzyme Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Mus musculus P05132
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 recombinant wild-type and mutant Y204A from Escherichia coli by ion exchange chromatography and gel filtration Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.11 ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate catalytic mechanism, active site structure, Tyr204 is required and involved in the hydrophobic network, substrate-induced interaction of Tyr204 and Asp166, overview Mus musculus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + Kemptide LRRASLG peptide substrate Mus musculus ADP + Kemptide phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Mus musculus