BRENDA - Enzyme Database show

Deoxyribonucleoside kinases belonging to the thymidine kinase 2 (TK2)-like group vary significantly in substrate specificity, kinetics and feed-back regulation

Knecht, W.; Petersen, G.E.; Munch-Petersen, B.; Piskur, J.; J. Mol. Biol. 315, 529-540 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.145
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as C-terminally His8-tagged protein in Escherichia coli strain BL21
Bombyx mori
2.7.1.145
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as full length and N-terminally truncated His-tagged enzymes in Escherichia coli
Xenopus laevis
2.7.1.145
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and as C-terminally His8-tagged protein in Escherichia coli strain BL21
Drosophila melanogaster
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
dCTP
competitive versus ATP, feedback inhibition
Xenopus laevis
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Bombyx mori
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Drosophila melanogaster
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Xenopus laevis
2.7.1.145
additional information
no inhibition by dNTPs other than dTTP
Bombyx mori
2.7.1.145
additional information
no inhibition by dNTPs other than dTTP
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
kinetics
Bombyx mori
2.7.1.145
additional information
-
additional information
kinetics
Xenopus laevis
2.7.1.145
0.0014
-
ATP
-
Bombyx mori
2.7.1.145
0.0025
-
ATP
-
Xenopus laevis
2.7.1.145
0.0061
-
2'-deoxycytidine
-
Bombyx mori
2.7.1.145
0.0153
-
2'-deoxythymidine
-
Bombyx mori
2.7.1.145
0.031
-
2'-deoxycytidine
-
Xenopus laevis
2.7.1.145
0.138
-
2'-deoxythymidine
-
Xenopus laevis
2.7.1.145
0.429
-
2'-deoxyguanosine
-
Bombyx mori
2.7.1.145
0.501
-
2'-deoxyadenosine
-
Bombyx mori
2.7.1.145
1.2
-
2'-deoxyguanosine
-
Xenopus laevis
2.7.1.145
2.1
-
2'-deoxyadenosine
-
Xenopus laevis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.145
additional information
no sorting signals in the amino acid sequence
Bombyx mori
-
-
2.7.1.145
nucleus
enzyme possesses 2 potential nuclear targeting sequences
Drosophila melanogaster
5634
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.145
Mg2+
-
Bombyx mori
2.7.1.145
Mg2+
-
Xenopus laevis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.145
26300
-
2 * 26300, recombinant enzyme, SDS-PAGE
Xenopus laevis
2.7.1.145
26900
-
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
2.7.1.145
29100
-
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
2.7.1.145
31300
-
2 * 31300, recombinant enzyme, SDS-PAGE
Bombyx mori
2.7.1.145
44600
-
recombinant N-terminally truncated enzyme, gel filtration
Drosophila melanogaster
2.7.1.145
46000
-
recombinant enzyme, native PAGE
Xenopus laevis
2.7.1.145
48500
-
recombinant full length enzyme, gel filtration
Drosophila melanogaster
2.7.1.145
49000
-
recombinant N-terminally truncated enzyme, native PAGE
Drosophila melanogaster
2.7.1.145
49800
-
recombinant enzyme, gel filtration
Xenopus laevis
2.7.1.145
57700
-
recombinant full length enzyme, native PAGE
Drosophila melanogaster
2.7.1.145
64400
-
recombinant enzyme, gel filtration
Bombyx mori
2.7.1.145
69300
-
recombinant enzyme, native PAGE
Bombyx mori
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.145
ATP + 2'-deoxycytidine
Xenopus laevis
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
Xenopus laevis
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Bombyx mori
Q9BKL3
putative
-
2.7.1.145
Drosophila melanogaster
-
-
-
2.7.1.145
Xenopus laevis
Q8UVZ9
putative
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.145
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
Bombyx mori
2.7.1.145
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, the His-tag is cleaved off by thrombin
Drosophila melanogaster
2.7.1.145
recombinant full length and N-terminally truncated His-tagged enzymes, the His-tags are cleaved off
Xenopus laevis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.145
brain
-
Xenopus laevis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
additional information
-
substrate specificity
Bombyx mori
2.7.1.145
additional information
-
substrate specificity
Xenopus laevis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
-
662607
Bombyx mori
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
-
662607
Xenopus laevis
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylthymine
-
662607
Bombyx mori
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylthymine
-
662607
Xenopus laevis
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-didehydro-3'-deoxythymidine
-
662607
Bombyx mori
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-dideoxycytidine
-
662607
Bombyx mori
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-dideoxycytidine
low activity
662607
Xenopus laevis
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-dideoxythymidine
-
662607
Bombyx mori
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-dideoxythymidine
low activity
662607
Xenopus laevis
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
662607
Bombyx mori
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
662607
Xenopus laevis
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
662607
Bombyx mori
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
662607
Xenopus laevis
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
662607
Bombyx mori
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
662607
Xenopus laevis
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
662607
Xenopus laevis
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
best substrate
662607
Xenopus laevis
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
high activity
662607
Bombyx mori
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2-chloro-2'-deoxyadenosine
low activity
662607
Xenopus laevis
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2-chloro-2'-deoxyadenosine
high activity
662607
Bombyx mori
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
low activity
662607
Bombyx mori
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
low activity
662607
Xenopus laevis
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 3'-deoxyadenosine
-
662607
Bombyx mori
ADP + 3'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 5-fluoro-2'-deoxyuridine
high activity
662607
Bombyx mori
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 5-fluoro-2'-deoxyuridine
high activity
662607
Xenopus laevis
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 9-beta-D-arabinofuranosyladenine
-
662607
Bombyx mori
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 9-beta-D-arabinofuranosyladenine
very low activity
662607
Xenopus laevis
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + aciclovir
-
662607
Bombyx mori
ADP + aciclovir phosphate
-
-
-
?
2.7.1.145
ATP + cytidine
-
662607
Bombyx mori
ADP + cytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + cytidine
very low activity
662607
Xenopus laevis
ADP + cytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + deoxyuridine
best substrate
662607
Bombyx mori
ADP + deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + deoxyuridine
high activity
662607
Xenopus laevis
ADP + deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + fludarabine
-
662607
Bombyx mori
ADP + fludarabine phosphate
-
-
-
?
2.7.1.145
ATP + stavudine
anti-HIV nucleoside analogue
662607
Bombyx mori
ADP + stavudine phosphate
-
-
-
?
2.7.1.145
ATP + uridine
very low activity
662607
Xenopus laevis
ADP + uridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + uridine
low activity
662607
Bombyx mori
ADP + uridine 5'-phosphate
-
-
-
?
2.7.1.145
additional information
substrate specificity
662607
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
662607
Bombyx mori
?
-
-
-
-
2.7.1.145
additional information
substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
662607
Xenopus laevis
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.145
dimer
2 * 31300, recombinant enzyme, SDS-PAGE
Bombyx mori
2.7.1.145
dimer
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
2.7.1.145
dimer
2 * 26300, recombinant enzyme, SDS-PAGE
Xenopus laevis
2.7.1.145
More
quarternary structure determination
Bombyx mori
2.7.1.145
More
quarternary structure determination
Xenopus laevis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.145
ATP
-
Xenopus laevis
2.7.1.145
ATP
-
Bombyx mori
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
inhibition kinetics, overview
Bombyx mori
2.7.1.145
additional information
-
additional information
inhibition kinetics, overview
Drosophila melanogaster
2.7.1.145
additional information
-
additional information
inhibition kinetics, overview
Xenopus laevis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as C-terminally His8-tagged protein in Escherichia coli strain BL21
Bombyx mori
2.7.1.145
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as full length and N-terminally truncated His-tagged enzymes in Escherichia coli
Xenopus laevis
2.7.1.145
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and as C-terminally His8-tagged protein in Escherichia coli strain BL21
Drosophila melanogaster
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.145
ATP
-
Xenopus laevis
2.7.1.145
ATP
-
Bombyx mori
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
dCTP
competitive versus ATP, feedback inhibition
Xenopus laevis
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Bombyx mori
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Drosophila melanogaster
2.7.1.145
dTTP
competitive versus ATP, feedback inhibition
Xenopus laevis
2.7.1.145
additional information
no inhibition by dNTPs other than dTTP
Bombyx mori
2.7.1.145
additional information
no inhibition by dNTPs other than dTTP
Drosophila melanogaster
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
inhibition kinetics, overview
Bombyx mori
2.7.1.145
additional information
-
additional information
inhibition kinetics, overview
Drosophila melanogaster
2.7.1.145
additional information
-
additional information
inhibition kinetics, overview
Xenopus laevis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
kinetics
Bombyx mori
2.7.1.145
additional information
-
additional information
kinetics
Xenopus laevis
2.7.1.145
0.0014
-
ATP
-
Bombyx mori
2.7.1.145
0.0025
-
ATP
-
Xenopus laevis
2.7.1.145
0.0061
-
2'-deoxycytidine
-
Bombyx mori
2.7.1.145
0.0153
-
2'-deoxythymidine
-
Bombyx mori
2.7.1.145
0.031
-
2'-deoxycytidine
-
Xenopus laevis
2.7.1.145
0.138
-
2'-deoxythymidine
-
Xenopus laevis
2.7.1.145
0.429
-
2'-deoxyguanosine
-
Bombyx mori
2.7.1.145
0.501
-
2'-deoxyadenosine
-
Bombyx mori
2.7.1.145
1.2
-
2'-deoxyguanosine
-
Xenopus laevis
2.7.1.145
2.1
-
2'-deoxyadenosine
-
Xenopus laevis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.145
additional information
no sorting signals in the amino acid sequence
Bombyx mori
-
-
2.7.1.145
nucleus
enzyme possesses 2 potential nuclear targeting sequences
Drosophila melanogaster
5634
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.145
Mg2+
-
Bombyx mori
2.7.1.145
Mg2+
-
Xenopus laevis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.145
26300
-
2 * 26300, recombinant enzyme, SDS-PAGE
Xenopus laevis
2.7.1.145
26900
-
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
2.7.1.145
29100
-
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
2.7.1.145
31300
-
2 * 31300, recombinant enzyme, SDS-PAGE
Bombyx mori
2.7.1.145
44600
-
recombinant N-terminally truncated enzyme, gel filtration
Drosophila melanogaster
2.7.1.145
46000
-
recombinant enzyme, native PAGE
Xenopus laevis
2.7.1.145
48500
-
recombinant full length enzyme, gel filtration
Drosophila melanogaster
2.7.1.145
49000
-
recombinant N-terminally truncated enzyme, native PAGE
Drosophila melanogaster
2.7.1.145
49800
-
recombinant enzyme, gel filtration
Xenopus laevis
2.7.1.145
57700
-
recombinant full length enzyme, native PAGE
Drosophila melanogaster
2.7.1.145
64400
-
recombinant enzyme, gel filtration
Bombyx mori
2.7.1.145
69300
-
recombinant enzyme, native PAGE
Bombyx mori
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.145
ATP + 2'-deoxycytidine
Xenopus laevis
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
Xenopus laevis
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
Bombyx mori
2.7.1.145
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, the His-tag is cleaved off by thrombin
Drosophila melanogaster
2.7.1.145
recombinant full length and N-terminally truncated His-tagged enzymes, the His-tags are cleaved off
Xenopus laevis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.145
brain
-
Xenopus laevis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
additional information
-
substrate specificity
Bombyx mori
2.7.1.145
additional information
-
substrate specificity
Xenopus laevis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
-
662607
Bombyx mori
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
-
662607
Xenopus laevis
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylthymine
-
662607
Bombyx mori
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 1-beta-D-arabinofuranosylthymine
-
662607
Xenopus laevis
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-didehydro-3'-deoxythymidine
-
662607
Bombyx mori
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-dideoxycytidine
-
662607
Bombyx mori
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-dideoxycytidine
low activity
662607
Xenopus laevis
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-dideoxythymidine
-
662607
Bombyx mori
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2',3'-dideoxythymidine
low activity
662607
Xenopus laevis
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
662607
Bombyx mori
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
662607
Xenopus laevis
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
662607
Bombyx mori
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
662607
Xenopus laevis
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
662607
Bombyx mori
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
662607
Xenopus laevis
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
662607
Xenopus laevis
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
best substrate
662607
Xenopus laevis
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
high activity
662607
Bombyx mori
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2-chloro-2'-deoxyadenosine
low activity
662607
Xenopus laevis
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2-chloro-2'-deoxyadenosine
high activity
662607
Bombyx mori
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
low activity
662607
Bombyx mori
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
low activity
662607
Xenopus laevis
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 3'-deoxyadenosine
-
662607
Bombyx mori
ADP + 3'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 5-fluoro-2'-deoxyuridine
high activity
662607
Bombyx mori
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 5-fluoro-2'-deoxyuridine
high activity
662607
Xenopus laevis
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 9-beta-D-arabinofuranosyladenine
-
662607
Bombyx mori
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 9-beta-D-arabinofuranosyladenine
very low activity
662607
Xenopus laevis
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + aciclovir
-
662607
Bombyx mori
ADP + aciclovir phosphate
-
-
-
?
2.7.1.145
ATP + cytidine
-
662607
Bombyx mori
ADP + cytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + cytidine
very low activity
662607
Xenopus laevis
ADP + cytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + deoxyuridine
best substrate
662607
Bombyx mori
ADP + deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + deoxyuridine
high activity
662607
Xenopus laevis
ADP + deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + fludarabine
-
662607
Bombyx mori
ADP + fludarabine phosphate
-
-
-
?
2.7.1.145
ATP + stavudine
anti-HIV nucleoside analogue
662607
Bombyx mori
ADP + stavudine phosphate
-
-
-
?
2.7.1.145
ATP + uridine
very low activity
662607
Xenopus laevis
ADP + uridine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + uridine
low activity
662607
Bombyx mori
ADP + uridine 5'-phosphate
-
-
-
?
2.7.1.145
additional information
substrate specificity
662607
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
662607
Bombyx mori
?
-
-
-
-
2.7.1.145
additional information
substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
662607
Xenopus laevis
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.145
dimer
2 * 31300, recombinant enzyme, SDS-PAGE
Bombyx mori
2.7.1.145
dimer
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE
Drosophila melanogaster
2.7.1.145
dimer
2 * 26300, recombinant enzyme, SDS-PAGE
Xenopus laevis
2.7.1.145
More
quarternary structure determination
Bombyx mori
2.7.1.145
More
quarternary structure determination
Xenopus laevis