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Literature summary extracted from

  • Park, S.H.; Kim, J.I.
    Characterization of recombinant Drosophila melanogaster myo-inositol-1-phosphate synthase expressed in Escherichia coli (2004), J. Microbiol., 42, 20-24.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.4 DNA sequence determination and analysis, expression of His-tagged INOS in Escherichia coli strain BL21(DE3) Drosophila melanogaster

General Stability

EC Number General Stability Organism
5.5.1.4 20% v/v glycerol, 2 mM PMSF, and 1 mM E-64 stabilize the native and the recombinant enzyme Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.4 additional information
-
additional information dissociation constants Drosophila melanogaster
5.5.1.4 0.42
-
NAD+ pH 7.5, 37°C, 7.5, recombinant enzyme Drosophila melanogaster
5.5.1.4 3.5
-
D-glucose 6-phosphate pH 7.5, 37°C, 7.5, recombinant enzyme Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.4 62000
-
4 * 62000, SDS-PAGE Drosophila melanogaster
5.5.1.4 271000
-
approximately, recombinant enzyme, gel filtration Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.4 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.4 recombinant His-tagged INOS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Drosophila melanogaster

Storage Stability

EC Number Storage Stability Organism
5.5.1.4 -20°C, purified recombinant enzyme, 20% v/v glycerol, completely stable for several weeks Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.4 D-glucose 6-phosphate
-
Drosophila melanogaster 1D-myo-inositol 3-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
5.5.1.4 tetramer 4 * 62000, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
5.5.1.4 iNOS
-
Drosophila melanogaster
5.5.1.4 Myo-inositol-1-phosphate synthase
-
Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.4 40
-
recombinant enzyme Drosophila melanogaster

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.5.1.4 20 50 increasing activity from 20°C to 40 °C, rapid loss of 60% activity at 50°C, inactive at 60°C Drosophila melanogaster

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.5.1.4 50
-
rapid loss of 60% activity Drosophila melanogaster
5.5.1.4 60
-
complete loss of activity after 5 min Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.4 7.5
-
recombinant enzyme Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.4 5 10 34% of maximal activity at pH 9.0, low activity below pH 5.0, inactive above pH 11.0 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
5.5.1.4 NAD+ required Drosophila melanogaster