EC Number | Cloned (Comment) | Organism |
---|---|---|
5.5.1.4 | DNA sequence determination and analysis, expression of His-tagged INOS in Escherichia coli strain BL21(DE3) | Drosophila melanogaster |
EC Number | General Stability | Organism |
---|---|---|
5.5.1.4 | 20% v/v glycerol, 2 mM PMSF, and 1 mM E-64 stabilize the native and the recombinant enzyme | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.4 | additional information | - |
additional information | dissociation constants | Drosophila melanogaster | |
5.5.1.4 | 0.42 | - |
NAD+ | pH 7.5, 37°C, 7.5, recombinant enzyme | Drosophila melanogaster | |
5.5.1.4 | 3.5 | - |
D-glucose 6-phosphate | pH 7.5, 37°C, 7.5, recombinant enzyme | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.5.1.4 | 62000 | - |
4 * 62000, SDS-PAGE | Drosophila melanogaster |
5.5.1.4 | 271000 | - |
approximately, recombinant enzyme, gel filtration | Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.5.1.4 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.4 | recombinant His-tagged INOS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Drosophila melanogaster |
EC Number | Storage Stability | Organism |
---|---|---|
5.5.1.4 | -20°C, purified recombinant enzyme, 20% v/v glycerol, completely stable for several weeks | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.4 | D-glucose 6-phosphate | - |
Drosophila melanogaster | 1D-myo-inositol 3-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.5.1.4 | tetramer | 4 * 62000, SDS-PAGE | Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.5.1.4 | iNOS | - |
Drosophila melanogaster |
5.5.1.4 | Myo-inositol-1-phosphate synthase | - |
Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.5.1.4 | 40 | - |
recombinant enzyme | Drosophila melanogaster |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.5.1.4 | 20 | 50 | increasing activity from 20°C to 40 °C, rapid loss of 60% activity at 50°C, inactive at 60°C | Drosophila melanogaster |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.5.1.4 | 50 | - |
rapid loss of 60% activity | Drosophila melanogaster |
5.5.1.4 | 60 | - |
complete loss of activity after 5 min | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.5.1.4 | 7.5 | - |
recombinant enzyme | Drosophila melanogaster |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.5.1.4 | 5 | 10 | 34% of maximal activity at pH 9.0, low activity below pH 5.0, inactive above pH 11.0 | Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.4 | NAD+ | required | Drosophila melanogaster |