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Literature summary extracted from

  • Van Overloop, H.; Gijsbers, S.; Van Veldhoven, P.P.
    Further characterization of mammalian ceramide kinase. Substrate delivery and (stereo)specificity, tissue distribution and subcellular localization studies (2006), J. Lipid Res., 47, 268-283.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.138 Ca2+ 1 mM, stimulatory effect in the absence of Mg2+ Homo sapiens
2.7.1.138 Mg2+ 3 mM, highly dependent on Mg2+ Homo sapiens
2.7.1.138 additional information (2R)-isomers of (2S)-substrates are stimulatory Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.138 expressed in CHO cells Homo sapiens
2.7.1.138 expressed in Escherichia coli Top10F cells Homo sapiens
2.7.1.138 expression in CHO cells in the plasma membrane Mus musculus
2.7.1.138 expression in Escherichia coli strain BL21(DE3), expression in CHO cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 additional information a truncated mutant enzyme, lacking the first 115 amno acids, is not active and fails to be located in the plasma membrane Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.138 3,3-cholamidopropyl-dimethylammonio-1-propanesulfonate almost complete inhibition at high concentrations Homo sapiens
2.7.1.138 Ca2+ above 1 mM; inhibition above 1 mM Homo sapiens
2.7.1.138 D-erythro-N,N-dimethylsphingosine
-
Homo sapiens
2.7.1.138 lauryldimethylammonium N-oxide inhibitory at low concentrations Homo sapiens
2.7.1.138 additional information no inhibition by ceramide analogues containing a carboxy group, i.e. N-acylated amino acids Homo sapiens
2.7.1.138 N-acetyl-D-erythro-sphingenine substrate inhibition above 0.1 mM Homo sapiens
2.7.1.138 N-laurylsarcosine inhibitory at low concentrations Homo sapiens
2.7.1.138 octyl-beta-D-glucopyranoside almost complete inhibition at high concentrations Homo sapiens
2.7.1.138 sphinganine
-
Homo sapiens
2.7.1.138 sphingenine
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.138 additional information
-
additional information kinetics of recombinant enzymes Homo sapiens
2.7.1.138 0.02
-
N-acetyl-D-erythro-sphingenine pH 7.2, 37°C, recombinant enzyme Homo sapiens
2.7.1.138 0.022
-
N-acetyl-sphingenine
-
Homo sapiens
2.7.1.138 0.03
-
N-hexanoyl-sphingenine
-
Homo sapiens
2.7.1.138 0.03
-
N-hexanoyl-D-erythro-sphingenine pH 7.2, 37°C, recombinant enzyme Homo sapiens
2.7.1.138 0.4
-
ATP in the presence of 3 mM Mg2+ Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.138 membrane
-
Homo sapiens 16020
-
2.7.1.138 membrane bound Mus musculus 16020
-
2.7.1.138 microsome
-
Mus musculus
-
-
2.7.1.138 microsome in brain and testis Rattus norvegicus
-
-
2.7.1.138 mitochondrion
-
Homo sapiens 5739
-
2.7.1.138 mitochondrion in testis Rattus norvegicus 5739
-
2.7.1.138 plasma membrane localization via the pleckstrin homology domain at the N-terminus Mus musculus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.138 Ca2+ stimulates to a lesser extent than Mg2+ Homo sapiens
2.7.1.138 Ca2+ 1 mM, stimulatory effect in the absence of Mg2+ Homo sapiens
2.7.1.138 Mg2+ at 3 mM MgCl2 Mus musculus
2.7.1.138 Mg2+ at 3 mM MgCl2 Rattus norvegicus
2.7.1.138 Mg2+ the recombinant enzyme is highly dependent on Mg2+, at 3 mM MgCl2, preferred divalent cation Homo sapiens
2.7.1.138 Mg2+ 3 mM, highly dependent on Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.138 60000
-
SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ATP + C16-ceramide Mus musculus
-
ADP + C16-ceramide 1-phosphate
-
?
2.7.1.138 ATP + C16-ceramide Homo sapiens
-
ADP + C16-ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide Mus musculus
-
ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide Rattus norvegicus
-
ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide Homo sapiens
-
ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + N-hexadecanoyl-sphingenine Homo sapiens
-
ADP + N-hexadecanoyl-sphingenine 1-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens Q8TCT0
-
-
2.7.1.138 Mus musculus
-
-
-
2.7.1.138 Mus musculus
-
male C57/B16 N mice
-
2.7.1.138 Rattus norvegicus
-
-
-
2.7.1.138 Rattus norvegicus
-
male Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.138 partially, subcellular fractionation Mus musculus
2.7.1.138 partially, subcellular fractionation Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.138 ATP + ceramide = ADP + ceramide 1-phosphate the secondary hydroxy group is not required for substrate recognition, preference for the D-erythro isomer, the enzyme is specific for th phosphorylation of the 1-hydroxy group Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.138 brain
-
Mus musculus
-
2.7.1.138 brain
-
Rattus norvegicus
-
2.7.1.138 cerebellum
-
Mus musculus
-
2.7.1.138 cerebellum high activity Homo sapiens
-
2.7.1.138 cerebrum
-
Mus musculus
-
2.7.1.138 cerebrum
-
Homo sapiens
-
2.7.1.138 heart high activity Mus musculus
-
2.7.1.138 liver
-
Rattus norvegicus
-
2.7.1.138 lung
-
Mus musculus
-
2.7.1.138 pancreas
-
Mus musculus
-
2.7.1.138 pancreas
-
Homo sapiens
-
2.7.1.138 skeletal muscle low activity Homo sapiens
-
2.7.1.138 spleen high activity Mus musculus
-
2.7.1.138 testis
-
Mus musculus
-
2.7.1.138 testis
-
Rattus norvegicus
-
2.7.1.138 testis high activity Mus musculus
-
2.7.1.138 testis high activity Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.138 0.92 5.4 recombinant enzyme in CHO cell extract, substrate N-C6-D-erythro-sphingenine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + C16-ceramide
-
Mus musculus ADP + C16-ceramide 1-phosphate
-
?
2.7.1.138 ATP + C16-ceramide
-
Homo sapiens ADP + C16-ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide
-
Mus musculus ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide
-
Rattus norvegicus ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide
-
Homo sapiens ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide in vitro substrate delivery of short chain ceramides requires bovine serum albumin, and a mixed micellar form consisting of octylglucoside and cardiolipin for long chain ceramides, choice of delivery system is important for in vitro activity Homo sapiens ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-erythro-N-hexanoyl-sphinganine
-
Homo sapiens ADP + D-erythro-N-hexanoyl-sphinganine 1-phosphate
-
?
2.7.1.138 ATP + D-erythro-N-hexanoyl-sphingenine
-
Homo sapiens ADP + D-erythro-N-hexanoyl-sphingenine 1-phosphate
-
?
2.7.1.138 ATP + N-acetyl-D-erythro-sphingenine
-
Homo sapiens ADP + N-acetyl-D-erythro-sphingenine 1-phosphate
-
?
2.7.1.138 ATP + N-acetyl-sphingenine
-
Homo sapiens ADP + N-acetyl-sphingenine 1-phosphate
-
?
2.7.1.138 ATP + N-hexadecanoyl-sphingenine
-
Homo sapiens ADP + N-hexadecanoyl-sphingenine 1-phosphate
-
?
2.7.1.138 ATP + N-hexadecanoyl-sphingenine very low activity Homo sapiens ADP + N-hexadecanoyl-sphingenine 1-phosphate
-
?
2.7.1.138 ATP + N-hexanoyl-1-O-hexadecyl-2-deoxy-2-amino-sn-glycerol
-
Homo sapiens ADP + ?
-
?
2.7.1.138 ATP + N-hexanoyl-D-erythro-sphingenine best substrate Homo sapiens ADP + N-hexanoyl-D-erythro-sphingenine 1-phosphate
-
?
2.7.1.138 ATP + N-hexanoyl-sphingenine
-
Homo sapiens ADP + N-hexanoyl-sphingenine 1-phosphate
-
?
2.7.1.138 ATP + N-tetradecanoyl-(2S)-amino-butan-1-ol
-
Homo sapiens ADP + ?
-
?
2.7.1.138 ATP + N-tetradecanoyl-(2S)-amino-hexadecan-1-ol
-
Homo sapiens ADP + ?
-
?
2.7.1.138 ATP + N-tetradecanoyl-2-amino-1-butanol
-
Homo sapiens ADP + ?
-
?
2.7.1.138 ATP + N-tetradecanoyl-2S-amino-1-butanol
-
Homo sapiens ADP + ?
-
?
2.7.1.138 additional information substrate specificity, stereochemistry Homo sapiens ?
-
?
2.7.1.138 additional information 2-amino-1-hexadecanol, 1-O-hexadecyl-2-deoxy-2-amino-sn-glycerol, N-tetradecanoyl-2(R)-pyrrolidine-methanol, N-tetradecanoyl-(R)-serine, N-tetradecanoyl-3-hydroxypyrrolidin, N-tetradecanoyl-4-amino-1-butanol, N-tetradecanoyl-3-amino-1-propanol, N-tetradecanoyl-(S)-serine, N-tetradecanoyl-2-benzylserine, N-tetradecanoyl3-phenylserine, N-tetradecanoyl-3-amino-tyrosine, N-tetradecanoyl-1-amino-2-naphthol-4-sulfonic acid, N-tetradecanoyl-3-hydroxy-anthranilic acid, N-tetradecanoyl-4-aminophenol, N-tetradecanoyl-diethanolamine, N-tetradecanoyl-ethanolamine, and N-tetradecanoyl-(S)-phenylalaninol are no substrates Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 CERK
-
Mus musculus
2.7.1.138 CERK
-
Rattus norvegicus
2.7.1.138 CERK
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.138 37
-
assay at Mus musculus
2.7.1.138 37
-
assay at Rattus norvegicus
2.7.1.138 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.138 6.2 7.8
-
Homo sapiens
2.7.1.138 7.2
-
assay at Mus musculus
2.7.1.138 7.2
-
assay at Rattus norvegicus
2.7.1.138 7.2
-
assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.138 ATP
-
Mus musculus
2.7.1.138 ATP
-
Rattus norvegicus
2.7.1.138 ATP
-
Homo sapiens