EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.138 | overexpression in HeLa cells, expression of His6-tagged enzyme in Spodoptera frugiperda Sf9 insect cells using the baculovirus infection system | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.138 | Ca2+ | at 3 mM | Homo sapiens | |
2.7.1.138 | Mg2+ | at 100 mM | Homo sapiens | |
2.7.1.138 | NaCl | at 50 mM | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.138 | ATP + ceramide | Homo sapiens | - |
ADP + ceramide 1-phosphate | - |
? | |
2.7.1.138 | additional information | Homo sapiens | the enzyme is a critical mediator of eicosanoid synthesis | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.138 | Homo sapiens | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.138 | additional information | - |
assay development utilizing Triton X-100 mixed micelles with cardiolipin, beta-octylglucoside, and diethylene pentaacetic acid | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.138 | ATP + 3-O-methyl C16:0 ceramide | very low activity | Homo sapiens | ADP + 3-O-methyl C16:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + ceramide | - |
Homo sapiens | ADP + ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + ceramide | stereospecific ceramide substrate recognition, the C4-5 trans double bond is important for substrate recognition, a minimum of 12C-acyl chain is required for activity | Homo sapiens | ADP + ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-erythro-C6:0 ceramide | very low activity | Homo sapiens | ADP + D-erythro-C6:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C10:0 ceramide | - |
Homo sapiens | ADP + D-eythro-C10:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C12:0 ceramide | - |
Homo sapiens | ADP + D-eythro-C12:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C14:0 ceramide | best substrate | Homo sapiens | ADP + D-eythro-C14:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C16:0 (R)-alpha-hydroxyceramide | low activity | Homo sapiens | ADP + D-eythro-C16:0 (R)-alpha-hydroxyceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C16:0 (S)-alpha-hydroxyceramide | very low activity | Homo sapiens | ADP + D-eythro-C16:0 (S)-alpha-hydroxyceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C16:0 ceramide | high activity | Homo sapiens | ADP + D-eythro-C16:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C16:0 dehydroceramide | - |
Homo sapiens | ADP + D-eythro-C16:0 dehydroceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C16:0 dihydroceramide | - |
Homo sapiens | ADP + D-eythro-C16:0 dihydroceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C16:0 urea ceramide | low activity, no activity with L-e-C16:0 urea ceramide | Homo sapiens | ADP + D-eythro-C16:0 urea ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C16:0-cis ceramide | low activity | Homo sapiens | ADP + D-eythro-C16:0-cis ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C18:0 ceramide | high activity | Homo sapiens | ADP + D-eythro-C18:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C18:0 phytoceramide | - |
Homo sapiens | ADP + D-eythro-C18:0 phytoceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C24:0 ceramide | - |
Homo sapiens | ADP + D-eythro-C24:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C24:1 ceramide | - |
Homo sapiens | ADP + D-eythro-C24:1 ceramide 1-phosphate | - |
? | |
2.7.1.138 | ATP + D-eythro-C8:0 ceramide | low activity | Homo sapiens | ADP + D-eythro-C8:0 ceramide 1-phosphate | - |
? | |
2.7.1.138 | additional information | the enzyme is a critical mediator of eicosanoid synthesis | Homo sapiens | ? | - |
? | |
2.7.1.138 | additional information | substrate specificity and stereochemistry, overview, methylation of the primary hydroxyl group results in loss of activity, the enzyme can use both ceramides with long saturated and long unsaturated fatty acyl chains | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.138 | CERK | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.138 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.138 | 7.2 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.138 | ATP | - |
Homo sapiens |