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Literature summary extracted from

  • Wijesinghe, D.S.; Massiello, A.; Subramanian, P.; Szulc, Z.; Bielawska, A.; Chalfant, C.E.
    Substrate specificity of human ceramide kinase (2005), J. Lipid Res., 46, 2706-2716.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.138 overexpression in HeLa cells, expression of His6-tagged enzyme in Spodoptera frugiperda Sf9 insect cells using the baculovirus infection system Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.138 Ca2+ at 3 mM Homo sapiens
2.7.1.138 Mg2+ at 100 mM Homo sapiens
2.7.1.138 NaCl at 50 mM Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ATP + ceramide Homo sapiens
-
ADP + ceramide 1-phosphate
-
?
2.7.1.138 additional information Homo sapiens the enzyme is a critical mediator of eicosanoid synthesis ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.138 additional information
-
assay development utilizing Triton X-100 mixed micelles with cardiolipin, beta-octylglucoside, and diethylene pentaacetic acid Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + 3-O-methyl C16:0 ceramide very low activity Homo sapiens ADP + 3-O-methyl C16:0 ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide
-
Homo sapiens ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + ceramide stereospecific ceramide substrate recognition, the C4-5 trans double bond is important for substrate recognition, a minimum of 12C-acyl chain is required for activity Homo sapiens ADP + ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-erythro-C6:0 ceramide very low activity Homo sapiens ADP + D-erythro-C6:0 ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C10:0 ceramide
-
Homo sapiens ADP + D-eythro-C10:0 ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C12:0 ceramide
-
Homo sapiens ADP + D-eythro-C12:0 ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C14:0 ceramide best substrate Homo sapiens ADP + D-eythro-C14:0 ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C16:0 (R)-alpha-hydroxyceramide low activity Homo sapiens ADP + D-eythro-C16:0 (R)-alpha-hydroxyceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C16:0 (S)-alpha-hydroxyceramide very low activity Homo sapiens ADP + D-eythro-C16:0 (S)-alpha-hydroxyceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C16:0 ceramide high activity Homo sapiens ADP + D-eythro-C16:0 ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C16:0 dehydroceramide
-
Homo sapiens ADP + D-eythro-C16:0 dehydroceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C16:0 dihydroceramide
-
Homo sapiens ADP + D-eythro-C16:0 dihydroceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C16:0 urea ceramide low activity, no activity with L-e-C16:0 urea ceramide Homo sapiens ADP + D-eythro-C16:0 urea ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C16:0-cis ceramide low activity Homo sapiens ADP + D-eythro-C16:0-cis ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C18:0 ceramide high activity Homo sapiens ADP + D-eythro-C18:0 ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C18:0 phytoceramide
-
Homo sapiens ADP + D-eythro-C18:0 phytoceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C24:0 ceramide
-
Homo sapiens ADP + D-eythro-C24:0 ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C24:1 ceramide
-
Homo sapiens ADP + D-eythro-C24:1 ceramide 1-phosphate
-
?
2.7.1.138 ATP + D-eythro-C8:0 ceramide low activity Homo sapiens ADP + D-eythro-C8:0 ceramide 1-phosphate
-
?
2.7.1.138 additional information the enzyme is a critical mediator of eicosanoid synthesis Homo sapiens ?
-
?
2.7.1.138 additional information substrate specificity and stereochemistry, overview, methylation of the primary hydroxyl group results in loss of activity, the enzyme can use both ceramides with long saturated and long unsaturated fatty acyl chains Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 CERK
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.138 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.138 7.2
-
assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.138 ATP
-
Homo sapiens