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Literature summary extracted from

  • Yamamoto, S.; Tsuzaki, Y.; Tougou, K.; Shinoda, S.
    Purification and characterization of L-2,4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus (1992), J. Gen. Microbiol., 138, 1461-1465.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.86 Co2+
-
 Acinetobacter calcoaceticus
4.1.1.86 Mn2+
-
 Acinetobacter calcoaceticus
4.1.1.86 Ni2+
-
 Acinetobacter calcoaceticus
4.1.1.86 Zn2+
-
 Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.86 1.51
-
 L-2,4-diaminobutanoate pH 8.5, 37°C Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.86 Ca2+ activates, preferred divalent cation Acinetobacter calcoaceticus
4.1.1.86 Mg2+ activates Acinetobacter calcoaceticus
4.1.1.86 additional information no effect by Na+ and K+ Acinetobacter calcoaceticus
4.1.1.86 Sr2+ activates Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.86 53000
-
 2 * 53000, SDS-PAGE Acinetobacter calcoaceticus
4.1.1.86 108000
-
 gel filtration Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate Acinetobacter calcoaceticus
-
 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate Acinetobacter calcoaceticus ATCC 23055
-
 propane-1,3-diamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.86 Acinetobacter calcoaceticus
-
 N-terminal amino acid sequence; strain ATCC23055
-
4.1.1.86 Acinetobacter calcoaceticus ATCC 23055
-
 N-terminal amino acid sequence; strain ATCC23055
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.86 native enzyme 2150fold to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and two steps of gel filtration Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.86 17.2
-
 purified enzyme Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate
-
 Acinetobacter calcoaceticus propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate specific for Acinetobacter calcoaceticus propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Acinetobacter calcoaceticus ATCC 23055 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate specific for Acinetobacter calcoaceticus ATCC 23055 propane-1,3-diamine + CO2
-
 ?
4.1.1.86 additional information no activity with 4-N-acetyl-L-2,4-diaminobutanoate, L-2,3-diaminopropionate, L-ornithine, and L-lysine Acinetobacter calcoaceticus ?
-
 ?
4.1.1.86 additional information no activity with 4-N-acetyl-L-2,4-diaminobutanoate, L-2,3-diaminopropionate, L-ornithine, and L-lysine Acinetobacter calcoaceticus ATCC 23055 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.86 dimer 2 * 53000, SDS-PAGE Acinetobacter calcoaceticus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.86 DABA DC
-
 Acinetobacter calcoaceticus
4.1.1.86 DABA decarboxylase
-
 Acinetobacter calcoaceticus
4.1.1.86 L-2,4-diaminobutyrate decarboxylase
-
 Acinetobacter calcoaceticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.86 45
-
-
 Acinetobacter calcoaceticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.86 40
-
 purified enzyme, stable for more than 15 min at pH 8.5 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.86 8.5 8.8
-
 Acinetobacter calcoaceticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.86 7.8 9.3 half maximal activity at pH 7.8 and pH 9.3 Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.86 pyridoxal 5'-phosphate maximal activity at 0.1 mM PLP, depletion leads to irreversible loss of 70% activity Acinetobacter calcoaceticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.86 Acinetobacter calcoaceticus isoelectric focusing
-
 5