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Literature summary extracted from

  • Wirtz, M.; Droux, M.; Hell, R.
    O-acetylserine (thiol) lyase: an enigmatic enzyme of plant cysteine biosynthesis revisited in Arabidopsis thaliana (2004), J. Exp. Bot., 55, 1785-1798.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 additional information
-
 additional information HPLC method for product quantification Arabidopsis thaliana
2.5.1.47 0.31
-
 O3-acetyl-L-serine isoform B, pH 7.5, 25°C, Michaelis-Menten kinetics Arabidopsis thaliana
2.5.1.47 0.32
-
 O3-acetyl-L-serine isoform B, pH 7.5, 25°C, Hill equation, Hill coefficient 1.03 Arabidopsis thaliana
2.5.1.47 0.43
-
 O3-acetyl-L-serine isoform C, pH 7.5, 25°C, Michaelis-Menten kinetics Arabidopsis thaliana
2.5.1.47 0.51
-
 O3-acetyl-L-serine isoform C, pH 7.5, 25°C, Hill equation, Hill coefficient 0.86 Arabidopsis thaliana
2.5.1.47 0.66
-
 O3-acetyl-L-serine isoform A, pH 7.5, 25°C, Hill equation, Hill coefficient 1.05 Arabidopsis thaliana
2.5.1.47 0.69
-
 O3-acetyl-L-serine isoform A, pH 7.5, 25°C, Michaelis-Menten kinetics Arabidopsis thaliana
2.5.1.47 3
-
 hydrogen sulfide isoform B, pH 7.5, 25°C, Michaelis-Menten kinetics Arabidopsis thaliana
2.5.1.47 3.2
-
 hydrogen sulfide isoform B, pH 7.5, 25°C, Hill equation, Hill coefficient 0.75 Arabidopsis thaliana
2.5.1.47 4.6
-
 hydrogen sulfide isoform C, pH 7.5, 25°C, Hill equation, Hill coefficient 1.05 Arabidopsis thaliana
2.5.1.47 4.7
-
 hydrogen sulfide isoform C, pH 7.5, 25°C, Michaelis-Menten kinetics Arabidopsis thaliana
2.5.1.47 5.6
-
 hydrogen sulfide isoform A, pH 7.5, 25°C, Michaelis-Menten kinetics Arabidopsis thaliana
2.5.1.47 6.4
-
 hydrogen sulfide isoform A, pH 7.5, 25°C, Hill equation, Hill coefficient 0.81 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.47 cytosol isoform A Arabidopsis thaliana 5829
-
2.5.1.47 mitochondrion isoform C Arabidopsis thaliana 5739
-
2.5.1.47 plastid isoform B Arabidopsis thaliana 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 additional information Arabidopsis thaliana model of a dynamic cysteine synthesis system with regulatory function ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Arabidopsis thaliana
-
 3 isoforms, recombinant enzymes, fast increase in enzyme product in response to sulfate deprivation
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 recombinant protein of isoforms A,B,C Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 550
-
 isoform C, pH 7.5, 25°C Arabidopsis thaliana
2.5.1.47 590
-
 isoform B, pH 7.5, 25°C Arabidopsis thaliana
2.5.1.47 900
-
 isoform A, pH 7.5, 25°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 additional information model of a dynamic cysteine synthesis system with regulatory function Arabidopsis thaliana ?
-
 ?
2.5.1.47 additional information preferred state of sulfide is hydrogen sulfide Arabidopsis thaliana ?
-
 ?
2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide
-
 Arabidopsis thaliana L-cysteine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 O-acetyl-L-serine (thiol) lyase
-
 Arabidopsis thaliana
2.5.1.47 OAS-TL
-
 Arabidopsis thaliana