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Literature summary extracted from

  • Wadgaonkar, R.; Nurmukhambetova, S.; Zaiman, A.L.; Garcia, J.G.
    Mutation analysis of the non-muscle myosin light chain kinase (MLCK) deletion constructs on CV1 fibroblast contractile activity and proliferation (2003), J. Cell. Biochem., 88, 623-634.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 expression of calmodulin-independent mutant MLCK1745 and of MLVKATPdel mutant in African green monkey kidney CV1 fibroblasts as V5-epitope- and His-tagged proteins Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.18 A27F/A29R/A31R/A32F mutant enzyme with defective ATP binding site, mutant leads to nearly unaltered MLC phosphorylation activity and altered morphology in transfected CV1 cells Homo sapiens
2.7.11.18 additional information construction of an enzyme mutant MLCK1745 which lacks the C-terminal amino acids including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, the MLCK1745 mutation leads to increased growth rates of transfected CV1 fibroblasts and an increase in basal level MLC phosphorylation compared to untransfected cells, stress fiber formation is unaffected by the mutation Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 190000
-
 x * 190000, recombinant enzyme mutant MLCK1745, SDS-PAGE, x * 210000, wild-type enzyme and mutant MLCKATPdel, SDS-PAGE Homo sapiens
2.7.11.18 210000
-
 x * 190000, recombinant enzyme mutant MLCK1745, SDS-PAGE, x * 210000, wild-type enzyme and mutant MLCKATPdel, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] Homo sapiens
-
 ADP + [myosin light chain] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 endothelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin light chain]
-
 Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain] activated phosphorylated enzyme Homo sapiens ADP + [myosin light chain] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 ? x * 190000, recombinant enzyme mutant MLCK1745, SDS-PAGE, x * 210000, wild-type enzyme and mutant MLCKATPdel, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 EC MLCK
-
 Homo sapiens
2.7.11.18 nmMLCK
-
 Homo sapiens
2.7.11.18 non-muscle myosin light chain kinase
-
 Homo sapiens
2.7.11.18 smMLCK
-
 Homo sapiens
2.7.11.18 smooth-muscle myosin light chain kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 37
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP residues A27, A29, A32, and A31 are involved in binding Homo sapiens
2.7.11.18 Calmodulin
-
 Homo sapiens