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Literature summary extracted from

  • Omura, H.; Ikemoto, M.; Kobayashi, M.; Shimizu, S.; Yoshida, T.; Nagasawa, T.
    Purification, characterization and gene cloning of thermostable o-acetyl-L-homoserine sulfhydrylase forming gamma-cyano-alpha-aminobutyric acid (2003), J. BIOSCI. BIOENG., 96, 53-58.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.49 expression in Escherichia coli Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 1.87
-
 O-acetyl-L-homoserine at 45°C, pH 7.5 Geobacillus stearothermophilus
2.5.1.49 4.17
-
 L-homocysteine at 45°C, pH 7.5 Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.49 CN-
-
 Geobacillus stearothermophilus
2.5.1.49 additional information cyanide ions can be replaced as cosubstrates by hydrogen sulfide, methylmercaptan, ethylmercaptan and phenylmercaptan Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.49 43000
-
 SDS-PAGE Geobacillus stearothermophilus
2.5.1.49 46850
-
 calculated from amino acid sequence Geobacillus stearothermophilus
2.5.1.49 180000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.49 o-acetyl-L-homoserine + CN- Geobacillus stearothermophilus glutamine and glutamic acid synthesis pathway gamma-cyano-alpha-aminobutyric acid + acetate
-
 ?
2.5.1.49 o-acetyl-L-homoserine + CN- Geobacillus stearothermophilus CN3 glutamine and glutamic acid synthesis pathway gamma-cyano-alpha-aminobutyric acid + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Geobacillus stearothermophilus
-
 isolated from a hot spring in Japan
-
2.5.1.49 Geobacillus stearothermophilus CN3
-
 isolated from a hot spring in Japan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 0.0272
-
 cell-free extract Geobacillus stearothermophilus
2.5.1.49 0.0479
-
 after heat treatment Geobacillus stearothermophilus
2.5.1.49 0.37
-
 after DEAE-cellufine A-500m treatment Geobacillus stearothermophilus
2.5.1.49 14.5
-
 after Phenyl-Toyopearl 650M treatment Geobacillus stearothermophilus
2.5.1.49 17.2
-
 after sephacryl S-200 HR treatment Geobacillus stearothermophilus
2.5.1.49 29.9
-
 recombinant enzyme purified from Escherichia coli Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 L-cysteine + KCN inactive Geobacillus stearothermophilus ?
-
 ?
2.5.1.49 L-cysteine + KCN inactive Geobacillus stearothermophilus CN3 ?
-
 ?
2.5.1.49 L-glutamic acid + KCN inactive Geobacillus stearothermophilus ?
-
 ?
2.5.1.49 L-glutamic acid + KCN inactive Geobacillus stearothermophilus CN3 ?
-
 ?
2.5.1.49 L-glutamine + KCN inactive Geobacillus stearothermophilus ?
-
 ?
2.5.1.49 L-glutamine + KCN inactive Geobacillus stearothermophilus CN3 ?
-
 ?
2.5.1.49 L-homocysteine + KCN
-
 Geobacillus stearothermophilus gamma-cyano-alpha-aminobutyric acid + H2S
-
 ?
2.5.1.49 L-homocysteine + KCN
-
 Geobacillus stearothermophilus CN3 gamma-cyano-alpha-aminobutyric acid + H2S
-
 ?
2.5.1.49 L-homoserine + KCN inactive Geobacillus stearothermophilus ?
-
 ?
2.5.1.49 L-serine + KCN inactive Geobacillus stearothermophilus ?
-
 ?
2.5.1.49 o-acetyl-L-homoserine + CN- glutamine and glutamic acid synthesis pathway Geobacillus stearothermophilus gamma-cyano-alpha-aminobutyric acid + acetate
-
 ?
2.5.1.49 o-acetyl-L-homoserine + CN- glutamine and glutamic acid synthesis pathway Geobacillus stearothermophilus CN3 gamma-cyano-alpha-aminobutyric acid + acetate
-
 ?
2.5.1.49 o-acetyl-L-homoserine + KCN
-
 Geobacillus stearothermophilus gamma-cyano-alpha-aminobutyric acid + acetate
-
 ?
2.5.1.49 o-acetyl-L-serine + KCN inactive Geobacillus stearothermophilus ?
-
 ?
2.5.1.49 o-phospho-L-serine + KCN inactive Geobacillus stearothermophilus ?
-
 ?
2.5.1.49 o-succinyl-L-homoserine + KCN inactive Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.49 monomer 1 * 43000, SDS-PAGE Geobacillus stearothermophilus
2.5.1.49 tetramer 4 * 43000, gel filtration, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.49 gamma-cyano-alpha-aminobutyric acid synthase
-
 Geobacillus stearothermophilus
2.5.1.49 o-acetyl-L-homoserine sulfhydrylase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.49 60
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.49 additional information
-
 no loss of activity after incubation at temperatures up to 60°C for 30 min at pH 7.5 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 8
-
 using Tris-HCl buffer Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.49 6 10.5 stable on incubation at 60°C for 30 min Geobacillus stearothermophilus