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Literature summary extracted from

  • Zhang, W.; Fletcher, L.; Muschel, R.J.
    The role of polo-like kinase 1 in the inhibition of centrosome separation after ionizing radiation (2005), J. Biol. Chem., 280, 42994-42999.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.21 expression in U2OS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.21 additional information Plk1 depletion by siRNA transfection combined with Nek2 overexpression in U2OS cells Homo sapiens
2.7.11.21 T210D site-directed mutagenesis, constitutively active mutant, overrides the irradiation-induced DNA damage and inhibition of centrosome separation in contrast to the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.21 additional information in vivo inhibition of Plk1 by DNA damage is ATM- or ATR-dependent Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.21 KCl
-
 Homo sapiens
2.7.11.21 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.21 additional information Homo sapiens the enzyme is involved in regulation of Nek2-induced centrosome separation after DNA damage ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.21 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.21 HeLa cell
-
 Homo sapiens
-
2.7.11.21 U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.21 ATP + casein
-
 Homo sapiens ADP + phosphorylated casein
-
 ?
2.7.11.21 additional information the enzyme is involved in regulation of Nek2-induced centrosome separation after DNA damage Homo sapiens ?
-
 ?
2.7.11.21 additional information the protein phosphatase 1 is no substrate of Plk1 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.21 Plk1
-
 Homo sapiens
2.7.11.21 Polo-like kinase 1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.21 30
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.21 ATP
-
 Homo sapiens