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Literature summary extracted from

  • Curien, G.; Ravanel, S.; Robert, M.; Dumas, R.
    Identification of six novel allosteric effectors of Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase isoforms. Physiological context sets the specificity (2005), J. Biol. Chem., 280, 41178-41183.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.4 L-alanine 13fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-alanine 3fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-cysteine 13fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-cysteine 3fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-isoleucine 13fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-isoleucine 3fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-leucine 4fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-serine 13fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-serine 3fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-valine 13fold activation at 2.5 mM Arabidopsis thaliana
2.7.2.4 L-valine 3fold activation at 2.5 mM Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.3 overexpression of AK-HSDH isoform 1 in Escherichia coli Arabidopsis thaliana
2.7.2.4 expression in Escherichia coli Arabidopsis thaliana

General Stability

EC Number General Stability Organism
2.7.2.4 pure protein , quickly frozen in N2 can be stored at -80°C for several months without any loss of activity Arabidopsis thaliana
2.7.2.4 pure protein, quickly frozen in N2 can be stored at -80°C for several months without any loss of activity Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.3 Thr
-
 Arabidopsis thaliana
2.7.2.4 L-leucine allosteric effector Arabidopsis thaliana
2.7.2.4 L-threonine in the presence of 1 mM aspartate and 2 mM ATP Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 0.42
-
 ATP 50 mM aspartate, presence of alanine Arabidopsis thaliana
2.7.2.4 0.48
-
 ATP 50 mM aspartate, presence of alanine Arabidopsis thaliana
2.7.2.4 1.5
-
 L-aspartate 20 mM ATP, presence of alanine Arabidopsis thaliana
2.7.2.4 2.2
-
 ATP 50 mM aspartate, absence of alanine Arabidopsis thaliana
2.7.2.4 2.3
-
 L-aspartate 20 mM ATP, presence of alanine Arabidopsis thaliana
2.7.2.4 2.6
-
 L-aspartate 20 mM ATP, absence of alanine Arabidopsis thaliana
2.7.2.4 6.15
-
 L-aspartate 20 mM ATP, absence of alanine Arabidopsis thaliana
2.7.2.4 6.5
-
 ATP 50 mM aspartate, absence of alanine Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.4 chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Arabidopsis thaliana
-
 ADP + 4-phospho-L-aspartate
-
 ?
2.7.2.4 ATP + L-aspartate Arabidopsis thaliana
-
 ADP + phospho-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Arabidopsis thaliana
-
-
-
2.7.2.4 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.3 recombinant enzyme Arabidopsis thaliana
2.7.2.4
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.4 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.3 L-aspartate 4-semialdehyde + NADPH
-
 Arabidopsis thaliana L-homoserine + NADP+
-
 ?
2.7.2.4 ATP + L-aspartate
-
 Arabidopsis thaliana ADP + 4-phospho-L-aspartate
-
 ?
2.7.2.4 ATP + L-aspartate
-
 Arabidopsis thaliana ADP + phospho-L-aspartate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.3 AK-HSDH isoform 1, bifunctional enzyme EC 2.7.2.4/EC 1.1.1.3 Arabidopsis thaliana
1.1.1.3 aspartate kinase-homoserine dehydrogenase bifunctional enzyme EC 2.7.2.4/EC 1.1.1.3 Arabidopsis thaliana
2.7.2.4 AK-HSDH I
-
 Arabidopsis thaliana
2.7.2.4 aspartate kinase I also acts as homoserine dehydrogenase (EC 1.1.1.3) Arabidopsis thaliana
2.7.2.4 aspartate kinase II also acts as homoserine dehydrogenase (EC 1.1.1.3) Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.4 0.049
-
 L-threonine in the presence of 1 mM aspartate and 2 mM ATP Arabidopsis thaliana
2.7.2.4 0.091
-
 L-threonine in the presence of 1 mM aspartate and 2 mM ATP Arabidopsis thaliana
2.7.2.4 1.1
-
 L-leucine
-
 Arabidopsis thaliana