Literature summary extracted from
Mok, M.T.; Edwards, M.R.
Kinetic and physical characterization of the inducible UDP-N-acetylglucosamine pyrophosphorylase from Giardia intestinalis (2005), J. Biol. Chem., 280, 39363-39372.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.23 |
native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli M15 |
Giardia intestinalis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.7.23 |
G108A |
dramatically reduced activity, significant increase in melting temperature |
Giardia intestinalis |
2.7.7.23 |
G210A |
dramatically reduced activity, significant decrease in melting temperature |
Giardia intestinalis |
General Stability
EC Number |
General Stability |
Organism |
---|
2.7.7.23 |
substrate binding increases enzyme stability against thermal inactivation |
Giardia intestinalis |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.7.7.23 |
0.03 |
- |
N-acetyl-alpha-D-glucosamine 1-phosphate |
purified recombinant native enzyme, pH 7.5, 37°C |
Giardia intestinalis |
|
2.7.7.23 |
0.038 |
- |
UTP |
Mg-UTP, purified recombinant native enzyme, pH 7.5, 37°C |
Giardia intestinalis |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.7.23 |
Mg2+ |
in complex with UTP |
Giardia intestinalis |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.7.23 |
47000 |
- |
gel filtration |
Giardia intestinalis |
2.7.7.23 |
49624 |
- |
1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration |
Giardia intestinalis |
2.7.7.23 |
50000 |
- |
1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration |
Giardia intestinalis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.7.7.23 |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate |
Giardia intestinalis |
involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis |
diphosphate + UDP-N-acetyl-D-glucosamine |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.23 |
Giardia intestinalis |
Q8MUP8 |
Portland 1 strain, ATCC30888 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.23 |
recombinant proteins using His-tag |
Giardia intestinalis |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.7.7.23 |
72 |
- |
purified recombinant native enzyme, pH 7.5, 37°C |
Giardia intestinalis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.23 |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate |
- |
Giardia intestinalis |
diphosphate + UDP-N-acetyl-D-glucosamine |
- |
? |
|
2.7.7.23 |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate |
involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis |
Giardia intestinalis |
diphosphate + UDP-N-acetyl-D-glucosamine |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.7.23 |
monomer |
1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration |
Giardia intestinalis |