Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mok, M.T.; Edwards, M.R.
    Kinetic and physical characterization of the inducible UDP-N-acetylglucosamine pyrophosphorylase from Giardia intestinalis (2005), J. Biol. Chem., 280, 39363-39372.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.23 native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli M15 Giardia intestinalis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.23 G108A dramatically reduced activity, significant increase in melting temperature Giardia intestinalis
2.7.7.23 G210A dramatically reduced activity, significant decrease in melting temperature Giardia intestinalis

General Stability

EC Number General Stability Organism
2.7.7.23 substrate binding increases enzyme stability against thermal inactivation Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.23 0.03
-
N-acetyl-alpha-D-glucosamine 1-phosphate purified recombinant native enzyme, pH 7.5, 37°C Giardia intestinalis
2.7.7.23 0.038
-
UTP Mg-UTP, purified recombinant native enzyme, pH 7.5, 37°C Giardia intestinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.23 Mg2+ in complex with UTP Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.23 47000
-
gel filtration Giardia intestinalis
2.7.7.23 49624
-
1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration Giardia intestinalis
2.7.7.23 50000
-
1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Giardia intestinalis involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis diphosphate + UDP-N-acetyl-D-glucosamine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.23 Giardia intestinalis Q8MUP8 Portland 1 strain, ATCC30888
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23 recombinant proteins using His-tag Giardia intestinalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.23 72
-
purified recombinant native enzyme, pH 7.5, 37°C Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Giardia intestinalis diphosphate + UDP-N-acetyl-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis Giardia intestinalis diphosphate + UDP-N-acetyl-D-glucosamine
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.23 monomer 1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration Giardia intestinalis