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Literature summary extracted from

  • Leonardi, R.; Chohnan, S.; Zhang, Y.M.; Virga, K.G.; Lee, R.E.; Rock, C.O.; Jackowski, S.
    A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation by coenzyme A (2005), J. Biol. Chem., 280, 3314-3322.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.33 additional information no feedback regulation by CoA or acetyl-CoA Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 gene coaA, expression of His6-tagged in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 additional information no feedback regulation by CoA or acetyl-CoA Staphylococcus aureus
2.7.1.33 N-heptylpantothenamide IC50 is 0.0048 mM, potent growth inhibitory anti-metabolite Staphylococcus aureus
2.7.1.33 N-pentylpantothenamide IC50 is 0.0035 mM, has antimicrobial activity against Staphylococcus aureus Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.003
-
 N-pentylpantothenamide pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
2.7.1.33 0.008
-
 N-heptylpantothenamide pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
2.7.1.33 0.023
-
 (R)-pantothenate pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
2.7.1.33 0.034
-
 ATP pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 Mg2+
-
 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.33 29000
-
 2 * 29000, recombinant enzyme, SDS-PAGE Staphylococcus aureus
2.7.1.33 59000
-
 recombinant enzyme, gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Staphylococcus aureus first step in coenzyme A biosynthesis ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate Staphylococcus aureus RN4220 first step in coenzyme A biosynthesis ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + N-heptylpantothenamide Staphylococcus aureus substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview ADP + N-heptylpantothenamide 4-phosphate
-
 ?
2.7.1.33 ATP + N-heptylpantothenamide Staphylococcus aureus RN4220 substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview ADP + N-heptylpantothenamide 4-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Staphylococcus aureus
-
 gene coaA
-
2.7.1.33 Staphylococcus aureus RN4220
-
 gene coaA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 recombinant His6-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.33 additional information
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Staphylococcus aureus ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate first step in coenzyme A biosynthesis Staphylococcus aureus ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Staphylococcus aureus RN4220 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate first step in coenzyme A biosynthesis Staphylococcus aureus RN4220 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + N-heptylpantothenamide
-
 Staphylococcus aureus ADP + N-heptylpantothenamide 4-phosphate
-
 ?
2.7.1.33 ATP + N-heptylpantothenamide substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview Staphylococcus aureus ADP + N-heptylpantothenamide 4-phosphate
-
 ?
2.7.1.33 ATP + N-heptylpantothenamide
-
 Staphylococcus aureus RN4220 ADP + N-heptylpantothenamide 4-phosphate
-
 ?
2.7.1.33 ATP + N-heptylpantothenamide substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview Staphylococcus aureus RN4220 ADP + N-heptylpantothenamide 4-phosphate
-
 ?
2.7.1.33 ATP + N-pentylpantothenamide
-
 Staphylococcus aureus ADP + N-pentylpantothenamide 4-phosphate
-
 ?
2.7.1.33 ATP + N-pentylpantothenamide
-
 Staphylococcus aureus RN4220 ADP + N-pentylpantothenamide 4-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.33 dimer 2 * 29000, recombinant enzyme, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 CoaA
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.33 37
-
 assay at Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.33 0.15
-
 N-pentylpantothenamide pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
2.7.1.33 0.425
-
 N-heptylpantothenamide pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
2.7.1.33 1.6
-
 ATP pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
2.7.1.33 1.7
-
 (R)-pantothenate pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 7.5
-
 assay at Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.33 ATP
-
 Staphylococcus aureus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.33 0.0035
-
 IC50 is 0.0035 mM, has antimicrobial activity against Staphylococcus aureus Staphylococcus aureus N-pentylpantothenamide
2.7.1.33 0.0048
-
 IC50 is 0.0048 mM, potent growth inhibitory anti-metabolite Staphylococcus aureus N-heptylpantothenamide