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Literature summary extracted from

  • Egelhoff, T.T.; Croft, D.; Steimle, P.A.
    Actin activation of myosin heavy chain kinase A in Dictyostelium: a biochemical mechanism for the spatial regulation of myosin II filament disassembly (2005), J. Biol. Chem., 280, 2879-2887.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 F-actin about 40fold activation of myosin heavy chain kinase A, structure and mechanism, stimulation via enzyme autophosphorylation Dictyostelium discoideum
2.7.11.7 G-actin slight activation of MHCK A, activation is abolished by latrunculin A, which depolymerizes the actin filament Dictyostelium discoideum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.7 overexpression of His-tagged full-length isozyme MHCK A and truncation mutant DELTAcoil-MHCK A comprising residues 1-498 in Dictyostelium discoideum, overexpression of isolated coiled-coil domain and of the catalytic domain in Escherichia coli as GST-fused proteins Dictyostelium discoideum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.7 additional information the truncation mutant DELTAcoil-MHCK A comprising residues 1-498 is not affected in its autophosphorylation rate by F-actin Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+
-
 Dictyostelium discoideum
2.7.11.7 KCl activates Dictyostelium discoideum
2.7.11.7 Mg2+
-
 Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + [myosin-heavy-chain] Dictyostelium discoideum
-
 ADP + [myosin-heavy-chain]phosphate
-
 ?
2.7.11.7 additional information Dictyostelium discoideum biochemical mechanism for the spatial regulation of myosin II filament disassembly acts via MHCK A activation by actin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum P42527 isozyme MHCK A; isozyme MHCK A
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.7 phosphoprotein MHCK A performs autophosphorylation, facilitated by F-actin requiring the coiled coil domain of the enzyme Dictyostelium discoideum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 recombinant His-tagged full-length isozyme MHCK A and truncation mutant DELTAcoil-MHCK A from Dictyostelium discoideum to homogeneity by affinity chromatography, recombinant GST-fusion coiled-coil domain and catalytic domain from Escherichia coli to near homogeneity by affinity chromatography Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + [myosin-heavy-chain]
-
 Dictyostelium discoideum ADP + [myosin-heavy-chain]phosphate
-
 ?
2.7.11.7 additional information biochemical mechanism for the spatial regulation of myosin II filament disassembly acts via MHCK A activation by actin Dictyostelium discoideum ?
-
 ?
2.7.11.7 additional information MHCK A performs autophosphorylation Dictyostelium discoideum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.7 MHCK A
-
 Dictyostelium discoideum
2.7.11.7 myosin heavy chain kinase A
-
 Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 25
-
 assay at Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 8
-
 assay at Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Dictyostelium discoideum