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Literature summary extracted from

  • Eyers, P.A.; Liu, J.; Hayashi, N.R.; Lewellyn, A.L.; Gautier, J.; Maller, J.L.
    Regulation of the G2/M transition in Xenopus oocytes by the cAMP-dependent protein kinase (2005), J. Biol. Chem., 280, 24339-24346.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP dependent on Xenopus laevis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.11 expression of N-terminally His6-tagged wild-type and mutant catalytic subunits C in Escherichia coli strain BL21(DE3) Xenopus laevis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.11 D328A site-directed mutagenesis of the catalytic subunit, mutant shows decreased interaction with the regulatory RI subunit Xenopus laevis
2.7.11.11 D328A/K72H site-directed mutagenesis of the catalytic subunit, inactive mutant showing decreased interaction with the regulatory RII subunit Xenopus laevis
2.7.11.11 K72H site-directed mutagenesis of the catalytic subunit, inactive mutant Xenopus laevis
2.7.11.11 K72R site-directed mutagenesis of the catalytic subunit, inactive mutant Xenopus laevis
2.7.11.11 additional information microinjection of mutant subunits C in Xenopus oocytes, overview Xenopus laevis
2.7.11.11 R133A site-directed mutagenesis of the catalytic subunit, mutant shows decreased interaction with the regulatory RII subunit Xenopus laevis
2.7.11.11 R133A/K72H site-directed mutagenesis of the catalytic subunit, inactive mutant showing decreased interaction with the regulatory RII subunit Xenopus laevis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 PKI recombinant His-tagged rabbit PKA inhibitor protein PKI, binding involves Arg133 Xenopus laevis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
 Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.11 additional information Xenopus laevis the enzyme regulates the G2/M transition in oocytes of Xenopus laevis, it also blocks progesterone-induced germinal vesicle envelope breakdown GVBD, Cdc-25-dependent dephosphorylation of Tyr15 in Cdc2, and synthesis of MEKK Mos ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 recombinant N-terminally His6-tagged wild-type and mutant catalytic subunits C from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.11 oocyte
-
 Xenopus laevis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + Kemptide peptide substrate, activity of catalytic PKA subunit Xenopus laevis ADP + phosphorylated Kemptide
-
 ?
2.7.11.11 additional information the enzyme regulates the G2/M transition in oocytes of Xenopus laevis, it also blocks progesterone-induced germinal vesicle envelope breakdown GVBD, Cdc-25-dependent dephosphorylation of Tyr15 in Cdc2, and synthesis of MEKK Mos Xenopus laevis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 More Arg133 is essential for binding of the catalytic subunit C to the regulatory subunit RII Xenopus laevis

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Xenopus laevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 30
-
 assay at Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.11 7.4
-
 assay at Xenopus laevis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Xenopus laevis