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Literature summary extracted from

  • Brand, L.A.; Strauss, E.
    Characterization of a new pantothenate kinase isoform from Helicobacter pylori (2005), J. Biol. Chem., 280, 20185-20188.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.33 additional information enzyme is not affected by CoA or acetyl-CoA Bacillus subtilis
2.7.1.33 additional information enzyme is not affected by CoA or acetyl-CoA Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 gene coaX, gene cluster analysis, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His6-tagged in Escherichia coli strain BL21(DE3) Bacillus subtilis
2.7.1.33 gene coaX, gene cluster analysis, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His6-tagged in Escherichia coli strain BL21(DE3) Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 additional information no inhibition by N-pentylpantothenamide, enzyme is not affected by CoA or acetyl-CoA Bacillus subtilis
2.7.1.33 additional information no inhibition by N-pentylpantothenamide, enzyme is not affected by CoA or acetyl-CoA Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.101
-
 (R)-pantothenate pH 7.6, 25°C Helicobacter pylori
2.7.1.33 0.168
-
 (R)-pantothenate pH 7.6, 25°C Bacillus subtilis
2.7.1.33 3.05
-
 ATP pH 7.6, 25°C Bacillus subtilis
2.7.1.33 9.59
-
 ATP pH 7.6, 25°C Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 KCl
-
 Bacillus subtilis
2.7.1.33 KCl
-
 Helicobacter pylori
2.7.1.33 Mg2+
-
 Bacillus subtilis
2.7.1.33 Mg2+
-
 Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Bacillus subtilis first step in coenzyme A biosynthesis ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate Helicobacter pylori first step in coenzyme A biosynthesis ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Bacillus subtilis
-
 gene coaX
-
2.7.1.33 Helicobacter pylori p37564 gene coaX
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus subtilis
2.7.1.33 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Helicobacter pylori ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate first step in coenzyme A biosynthesis Bacillus subtilis ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate first step in coenzyme A biosynthesis Helicobacter pylori ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate the enzyme is absolutely specific for ATP, no activity with CTP, GTP, UTP, or phosphoenolpyruvate as phosphoryl donors Bacillus subtilis ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 CTP + (R)-pantothenate
-
 Helicobacter pylori CDP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 GTP + (R)-pantothenate
-
 Helicobacter pylori GDP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 additional information N-pentylpantothenate is no substrate Bacillus subtilis ?
-
 ?
2.7.1.33 additional information N-pentylpantothenate is no substrate, no activity with UTP or phosphoenolpyruvate as phosphoryl donors Helicobacter pylori ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 CoaX
-
 Bacillus subtilis
2.7.1.33 CoaX
-
 Helicobacter pylori
2.7.1.33 More the enzyme forms a type III pantothenate kinase Bacillus subtilis
2.7.1.33 More the enzyme forms a type III pantothenate kinase Helicobacter pylori
2.7.1.33 PanK
-
 Bacillus subtilis
2.7.1.33 PanK
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.33 25
-
 assay at Bacillus subtilis
2.7.1.33 25
-
 assay at Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.33 2.09
-
 ATP pH 7.6, 25°C Helicobacter pylori
2.7.1.33 2.09
-
 (R)-pantothenate pH 7.6, 25°C Helicobacter pylori
2.7.1.33 2.12
-
 ATP pH 7.6, 25°C Bacillus subtilis
2.7.1.33 2.12
-
 (R)-pantothenate pH 7.6, 25°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 7.6
-
 assay at Bacillus subtilis
2.7.1.33 7.6
-
 assay at Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.33 ATP absolutely specific for Bacillus subtilis
2.7.1.33 ATP can partially be substituted by CTP or GTP Helicobacter pylori
2.7.1.33 CTP less active than ATP Helicobacter pylori
2.7.1.33 GTP less active than ATP Helicobacter pylori