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Literature summary extracted from

  • Zhu, H.; Shuman, S.
    Structure-guided mutational analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) (2005), J. Biol. Chem., 280, 12137-12144.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.2 D117E 3% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 D117N less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 D138A 37% of ligase activity of wild-type enzyme Escherichia coli
6.5.1.2 D283A 57% of ligase activity of wild-type enzyme Escherichia coli
6.5.1.2 D285E 4% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 D285N less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 E143A 52% of ligase activity of wild-type enzyme Escherichia coli
6.5.1.2 E173A less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 E173D less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 E173Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 G118A less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 G172A 36% of ligase activity of wild-type enzyme Escherichia coli
6.5.1.2 G286A 14% of ligase activity of wild-type enzyme Escherichia coli
6.5.1.2 K115Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 K115R less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 K290A 13% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 K314Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 K314R 5% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 L119A 110% of ligase activity of wild-type enzyme Escherichia coli
6.5.1.2 N198A 26% of ligase activity of wild-type enzyme Escherichia coli
6.5.1.2 R200A less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 R200K less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 R200Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 R208A 2% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 R208K 1.2% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 R208Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 R277A 1.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
6.5.1.2 R277A rate of isolated sealing step is 20% of the activity of wild-type enzyme Escherichia coli
6.5.1.2 R277K 5.6% of ligase activity of wild-type enzyme, themperature-sensitive mutation, rate of isolated sealing step is 4% of the activity of wild-type enzyme Escherichia coli
6.5.1.2 R277Q 1.8% of ligase activity of wild-type enzyme, lethal mutation, rate of isolated sealing step is 10% of the activity of wild-type enzyme Escherichia coli
6.5.1.2 V288A 74% of ligase activity of wild-type enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 LigA
-
 Escherichia coli
6.5.1.2 NAD+-dependent DNA ligase
-
 Escherichia coli